When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. Details. 001/min at 425 nm. 7. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. The SPECTRAmax 340PC can mea-The extracted stain was then transferred to a 96-well plate, and the absorbance at 562 nm was measured using a SpectraMax 340 plate reader/spectrophotometer(Molecular Devices Corp. 0, 20 mmol L –1cal-cium chloride). Absorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. After washing, the dye was eluted with 3% HCl, and the absorbance measured with a SpectraMax 340 microtiter plates reader at 665 nm. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode Opening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. Following incubation for 30 min at room temperature, the reaction was quenched by addition of 100 μl stop solution (2 N H 2 SO 4) and absorbance was read using microplate reader (SpectraMax 340; Molecular Devices Co. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Upgradeable modules are also available including western blot, cell imaging, and fast. 5, 2 mM pNPP, and recombinant enzymes, PTP1B activities were continuously monitored on a SpectraMax 340 Microplate Reader (SpectraMax, Silicon Valley, CA, USA) at 405 nm for 2 min at 30°C. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that. Molecular Devices SpectraMax M5e Microplate Reader. Plasma glucose levels were measured using FreeStyle Lite (Abbott Diabetes Care Ltd. Endpoint, kinetic, spectrum. Integration delay. ): 8. 2. The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). The absorbance was measured at 340 nm for 2 min at a 15 s time interval. The plate was read on a SpectraMax 340 PC plate reader (Molecular Devices, Sunnyvale, CA) at 450 nm, with reference at 650 nm. Immunofluorescence and Aggregation Analysis. Samples with OD values of less than 0. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). Near bottom. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance plate reader (Molecular Devices, Union City, CA). Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid- plates can be used in the SPECTRAmax PLUS 384. Statistical analysis. Not. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. One plate carrier adapter is provided with the instrument. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. ScanLater Western Blot Detection Cartridge. Wavelength(s) 340 to 850 nm; Add to Compare List. Immunofluorescence and Aggregation Analysis. 2. Or scan up to 384 wells at any time to determine the best assay conditions. Also for: Spectramax 190, Spectramax 340pc384, Versamax. 3. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. (7. Tunable for filterless flexibility. Male 8 pin mini Din to Female 9D. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. FTIR Spectrometer: Shimadzu IRSpirit (2018)The data were acquired with a Molecular Devices SpectraMax 340 Microplate Reader (Sunnyvale, CA, USA). Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). High fg levels of Streptavidin High fg levels of Streptavidin Exceeds Cisbio certification requirements TRF-based with 340/80 nm EX and 616/10 nm EM AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm. 5. 94 g calcium chloride dihydrate (both. 5. The antibodies originally defining this molecule by inducing apoptosis, anti-Fas and anti-APO-1 , were of the IgM or IgG3 subtype, respectively. 0% and ± 0. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). 5 mL of 1 M acetate buffer (pH 4. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Dissolve 13. Products : SpectraMax Plus 384 reader,. Warranty. 306 plate air cal. Awareness Technology Inc. S. 0200-0729. , Sunnyvale, CA). Number of flashes. , Sunnyvale, CA). Fully tested with WarrantyThe SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). This test was performed in duplicates. Spectrophotometer (SpectraMax 340). Incubation with TMB substrate was done for 18 min. , Fremont, CA, USA). 4. 30. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. or microplate reader (e. Unit has several areas of damaged plastic. Bioz Stars score: 86/100, based on 1 PubMed citations. The OD for each serum is adjusted by subtracting either the OD for spontaneous mediator release or antigen-specific mediator release. ). EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. 0200-6117. Key benefits • User-upgradeable application modules including cellular imaging SpectraTest ABS1 Absorbance Validation Plate. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Molecular Devices SpectraMax M5e Microplate Reader. Following cell culture under normoxic or hypoxic con-ditions, culture medium samples were obtained on completing the incubation period to measure atrial natriuretic peptide. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. reader (SpectraMax 340; Molecular devices, LLc). The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. 2 were considered negative for IgM antibody. Call 1-800-745-2710. , Sunnyvale, CA). Samples containing 25 μg of protein were separated on 12. Summary of Contents for Molecular Devices SPECTRAmax 340PC. Oxidative stress induction by H 2 O. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. SpectraMAX 340 plate reader (Molecular Devices). automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. Or scan up to 384 wells at any time to determine the best assay conditions. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. using SpectraMax i3 (Molecular devices, San Jose, USA). Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. Permeability of the monolayer was verified by adding 1% Evans Blue dye (Thermo Fisher Scientific, Inc. 0 0 The indicated microplates were scanned form 200 nm to 800 nm. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross awiderangeofconcentrations. Our absorbance plate readers feature our PathCheck Sensor technology and. Molecular Devices’ PathCheck® sensor technology. Although all samples initially were analyzed at the same volume, the volume of sample. With SOFTmax PRO, the contents of the wells in a microplate can be mixed used in the SPECTRAmax PLUS. Filipin StainingSpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. After 72 h, 20 μl of 5 mg/ml MTT solution was added to each well and plates were incubated for 3 h at 37°C. They are for reference only. measurements were performed on a SPECTRAmax-340 plate reader (Molecular Devices Inc. Isolation of RNA and RT-PCR. Subsequent replacements require only the lamp (N-34-LB) be replaced. Manager: Yinghua Chen, PhD Contact Email: yinghua. Application note showing how SpectraMax® i3x and SpectraMax®iD5 Multi-Mode Microplate Readers are used to performGq-coupled receptor assays using HTRF technology. edu Contact Phone: 216-368-8962. Screening for Antigen-Specific IgG Producing Clones by Capture ELISA. This was followed by an additional incubation at 37 °C for 30–60 min. 4; 100 m M NaCl, 0. 5 hours, and is measured in a 96-well plate spectrophotometer at 405 nm (SpectraMax 340; Molecular Devices LLC, Sunnyvale, CA, USA). 30. SpectraMAX 340 microtiter plate reader. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). PC12 or BV-2 cells treated with various concentrations of BBD were stressed with hypoxia for one hour and the supernatant was then assayed for LDH activity. The reduction of HED in the presence of NADPH and GR was recorded at 340 nm. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. Call 1-800-745-2710. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Please see photos. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays. Multi-mode Microplate Readers. Wells were washed three times. 304 signal level saturation. Cet appareil ISM est confomre à la norme NMB-001 du Canada. The GH Pro 340 Supplemental Greenhouse LED grow lighting is designed with greenhouse operations in mind. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 4 0112-0126D SafetyInformation. 25–0. SpectraMax QuickDrop Micro-Volume Spectrophotometer. 30 µs. Following incubation, 100 μl of solution was transferred to new wells and the color change in the solution was measured with a microtiter plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. 06 ng/mL; FSH: 0. VersaMax reads 96-well and strip-well plates. One plate carrier adapter is provided with the instrument. Cross sections of mouse skin was prepared and stained as previously described . Fibrin clots were formed as described above with some modifications 46. This is equivalent to having 511 built-in filters,Results: A total of 64% of the subjects reported improved quality of sleep during treatment with naloxone at a dose of 40 ng per 24 hours as compared with 9% with sham treatment (P=0. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). Cell proliferation was measured using the MTT method. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. Title: SPECTRAmax 340 Microplate Reader manual Author: Research Facilities Keywords: spectramax, 340, microplate reader, manual, Created Date: 12/13/2005 9:07:53 AMSpectraMax Multi-Mode Microplate Readers. 3 0. Not all manufacturers' microplates are the same with regard to design, materials, orAbsorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). SpectraMax 190 reads 96-well and strip-well plates. Acceptor: 665/10 nm. The SpectraMax® iD3 Multi Mode Microplate . Reagena Poc Puumala® was used for detection of IgM antibody against PUUV in NE patient sera at the Department of Clinical Virology,. The optical density at 405 nm (OD 405) of the plates was measured with a microplate reader (SpectraMax 340 PC384, Molecular Devices) before and after a 1-h incubation at room temperature. The loading equaled the mass of BSA per mass of particles. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. No power cord or accessories included. 05 ng/mL; progesterone: 2. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 570nm on the indicated days (Cell Proliferation Kit I; Roche). Like CD3, the CD95 death receptor requires binding of immobilized or multimeric antibodies to be activated effectively. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. . albicans pre. 04. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Each experiment was performed in duplicate. 0. The activity of glutathione peroxidase (GPX) was quantified spectrophotometrically at 340 nm, following the method of Takeda et al. Cell viability was determined using the blue formazan assay in which colorless 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is metabolized to a blue product by mitochondrial dehydrogenases. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. The Spectra Catalina 340z produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize yacht with two to four people onboard. HTRF-compatible SpectraMax Multi-Mode Readers. For more sample throughput, the system canThe optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. SpectraMax Microplate Readers. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. ), which was proportional to the number of damaged cells. Microplate Reader Shelf. SpectraMAX 340. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. Samples were considered “positive” if the absorbance value was greater than two times the average absorbance value of the negative control wells . Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. 024). This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). Details. Accurate quantitation The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)automated SpectraMAX 340 microtiter plate reader. Oxidative stress induction by H 2 O 2 and intracellular ROS analysis in L-929 cells. Analysis of ALA by Ehrlich’s Reagent Assay. A 0. Stat Fax 4700 Microstrip Reader. Optical density was measured using a SpectraMAX 340 (Molecular Devices, Sunnyvale, CA). Percent viability was calculated by comparison with the control group. The extreme flexibility and high sensitivity of the SpectraMax GEMINI EM make it appropriate for applications within the fields of bio-A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 7 macrophages were obtained from ATCC (Manassas, VA). Vessel Sprout Formation Assay. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. control. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 2. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. 8 (w) x 15 (d) Performance Specifications Wavelength Range: 340 – 850 nm Wavelength Selection: Monochromator, tunable 1. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Adhesion molecule expression To determine whether baicalein could modify the oxLDL-induced adhesion molecule expression, HUVECs were grown to confluence, pretreated with baicalein for 2 hrs and stimulated with oxLDL (150 μg/ml) for. ELISAs were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). 2 0. 4. The spectrophotometers used were SpectraMax 340 (for GSH, GCS, and HNE-His assays) and SpectraMax M3 (for GGT, GPx, and GST assays), both from Molecular Devices, Sunnyvale, CA. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. The amount of liberated pNA product during incubations was determined using a standard curve obtained at 410 nm for various concentrations of a pNA stock solution in the SpectraMax 340 microplate reader. Then the samples were incubated for 1 h with human IL6 detecting antibody and then with HRP conjugate for 40 min. The plate was covered and incubated for one hour at room temperature. SpectraMax Multi-Mode Microplate Readers. TDP-43 stress granular cells were treated with 70% ice-cold. The extinction coefficient at 280 nm used for determining the concentration of Rsp3690 was calculated to be 28,795 M –1 cm –1. 2 g/L solution; dilute 1:10 in HBSS. SpectraMax ® Plus 384 optics. What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full bandwidth at 1/2 the transmission peak for the selected wavelength. control or celatrol-treated cells. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. 313 reference gain check fail. Quick View. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Absorbance values were measured using a plate reader (Molecular Devices; SpectraMax 340) at λ = 405 nm. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. Molecular Devices SpectraMAX 340 Microplate Reader. CD154 is an established indicator of antigen-reactive T helper cells in other species, but has not been characterized in horses. 0, MSD) or Spectramax 340 (Molecular Devices). Although not statistically significant, pain was reduced by 2 NRS steps or more during supplemental treatment with naloxone in 36% of subjects when. Microplate Readers. The optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. The absorbance values were measured at 450 nm using a SpectraMax® 340 plate reader (Molecular Devices, Sunnyvale, CA, USA). For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. TheSpectraMax®190MicroplateSpectrophotometer,SpectraMax®340PC384Microplate Spectrophotometer,andVersaMax™MicroplateSpectrophotometerproviderapidandsensitive. 2 software was used to measure and calculate of the bioactivity activity studies. 0 to 4. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). 305 reference level saturation. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. 0% and ± 0. The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. The plates were incubated for 4 additional hours at 37°C. 5 ng/ml; estradiol: 10 pg/ml. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. 0 3. Molecular Devices SpectraMax Gemini XS Microplate Reader. Customer Transformation Hub. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of. D2. 5. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. 4- to 5-weeks old female BALB/c mice were purchased from Charles River Laboratories (QC, Canada). Figure 1. 8. Quick View. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. FXII or PK activation by FXIa was tested as follows: FXII or PK (200nM) was incubated with or without FXIa (25nM) in TBS, 0. Novak, Nathan J. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). These robust readers have been placed in labs from Antarctica to the. When necessary, samples were. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. The narrow bandwidth used by the model increases measurement accuracy and broad analysis over. An absorbance was read at 490/630 nm using a spectraMAX 340 microtiter plate reader. The SpectraMax 384 Plus lets you get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. Description. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices). All strains were tested three times and the results averaged. Cells were scraped from the plate and single cell suspensions were made by passing cells through 28G1/2 insulin syringes. The ratio of red to green fluorescence intensity was calculated. The relative LDH release was determined as the percentage ratio of the optical densities in the media (containing CNMs at each. 5 stars - 1602 reviewsThis assay typically develops in 1. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. Setup recommendations for SpectraMax. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the visible range. ” SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). See More Microplate Reader / Microtiter Plate Reader. 5 mL sample or standard solution was well mixed with 0. edu Contact Phone: 216-368-8962. All assays were performed in triplicate, and the absorbance at 405 nm was measured using a SpectraMax 340 multiwell plate reader (Molecular Devices). ). Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). Quick View. 1 mL of acetylacetone. Pharmacokinetic and pharmacodynamic analysis. Continuous kinetic monitoring of enzyme activity was performed on SPECTRAmax 340 or Flexstation 2-384 microplate reader (Molecular Devices, Sunnyvale, CA, USA) and controlled by the Softmax software. The metals were preincubated with A for 5 min when added before heme. Details. Fios Router Setup Manual, Losi Xx4 We Manual, Statics Hibbeler Solutions Manual 12th Edition, Nokia Hs 23 Manual, Spectramax 340 Manual, Nolan N102 User Manual, Ship Security Officer Manual yunqian 4. Includes: Computer Loaded with Softmax Pro 5. . The assays contained 0. SpectraMax 384 Plus. We also offer depot Repair Service from our base of operations in Southern California (San Diego). SPECTRAMAX 340. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideHigh-throughput assays for promiscuous inhibitors Brian Y Feng1,3, Anang Shelat1,3, Thompson N Doman2,RKipGuy1 & Brian K Shoichet1 High-throughput screening (HTS) searches large libraries of chemical compounds. The sensitivities of the hormones are Prolactin: 0. Statistical analysis The significant differences between the different strains in the adherence assays were analyzed using the Student’s t -test. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Call 1-800-745-2710. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. IMAP assays are based on binding of phosphate to immobilized metal coordination complexes on nanoparticles. The concentration of TNFα in the experimental samples was calculated from a mouse TNFα standard curve of 15–2000 pg/ml. Labsystems Luminoskan RT Microplate Reader. SpectraMax® M2/M2e user guide a multi-detection microplate reader with two-mode cuvette port SpectraMax M2e user guide cover 1 1 4/21/06 9:53:28 AM. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Amino Acid Analysis. Filterless Absorbance Microplate Readers provides fast & consistent results with steady temperature enabling a wide range of assays - ELISA reader, microbial growth, & protein quantitation. Liquid handling for random screening was carried out with a Biomek FX from Beckman Coulter (Fullerton, CA, USA) and HYTRA. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. It measures the optical density (OD) of samples in both 96- and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time. The VersaMax and SpectraMax 190 read 96-well microplates. Irene Tieleman, Ido PenandSimon VerhulstSubsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). Cell Culture AO391 (derived from human aorta) and CD314 (derived from human carotid artery) cell lines were a generous gift from Wendy Bauer (Tufts Molecular Cardiology Research Institute) and were grown in DMEM (CellGro) supplemented with 10% FBS. Features demonstrated by the model include temperature control, a robot-compatible interface and data analysis software. Example: [Drawer]. In vivo anti-malarial assay in mice. Photometric Range: 0. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. RESEARCH ARTICLE Effects of early-life conditions on innate immune function in adult zebra finches Merijn M. Please refer to photos for further. Spectral Range. After treatment, total cellular RNA was extracted with a cold RNA extraction solution (Ultraspec RNA;Call 1-800-745-2710. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. reader (Molecular Devi ces). The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance. Nitric Oxide Assay. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating. One plate carrier adapter is provided with the instrument. Stat Fax 3200 Microplate Reader. 0 1. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . TheSpectraMax®Plus384addstheabilitytoread SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Fig. Scanning was done at 600 nm, using a Molecular Devices SpectraMax 340 plate reader. 0. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. SPECTRAmax® 190. ) to the upper chamber, followed by measurement with a spectrophotometer (SpectraMax 340, Molecular Devices LLC, Sunnyvale, CA, USA) at 610 nm of media in the lower chamber after one hour . 003 OD Stray light ≤ 0. Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. , Sunnyvale, CA, USA) at a wavelength of 450 nm. Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. 0200-7027. Examples: “Press carefully to engage,” “Do not press down. Each experiment was performed in duplicate and repeated at. Detection limits. SPECTRAmax™ 340 (Molecular Device, CA, USA) at 37 ° C for 30 min. FlexStation® 3 Multi-Mode Microplate Reader. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. Glutathione reductase (GR) activity was measured, according to Smith et al. StakMax. FACS analysis. AoSMCs were seeded into 6-well culture plates.