This protocol gives the cells viability and confluence to be monitored. FOR HTRF CERTIFIED. MATERIALS PROVIDED: KIT . FOR HTRF CERTIFIED READER. 96-well culture plateFOR HTRF CERTIFIED READER For more information about HTRF. For more information about HTRF ® compatible readers and for set-up recommendations, please. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Remove the plate sealer and read on an HTRF® compatible reader . Alpha Technology: The VICTOR Nivo system. VICTOR Nivo Multimode Plate Reader • Compact and lightweight • High performance for routine applications, including biochemical and cell-based assays • All major detection. 5 µL. Remove the plate seal and read on an HTRF compatible reader. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Please note that this kit use a Terbium donor crypate, make sure to set up your reader for Terbium cryptate. Manufacturer. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. For more information about HTRF ®Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. They can be used in a no-transfer. Highly compatible plates for HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. • Low volume (20µl) • Compatible with all HTRF readers • For cell-based or biochemical assays Trying is believing. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4 SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. Make sure the setup for Eu3+ Cryptate is used. Customer Transformation Hub. For more information about HTRF. Lysates are then transferred to the assay plate for the detection of total-Rb by HTRF. Even if not listed, some readers may be compatible with HTRF technology. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. Excitation lasers significantly improve performance and lower. At Cisbio we provide microplates that help you discover better. Plate 25 μL of cells in. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Even if not listed, some. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. PLATE READER INTRODUCTION Selecting the right plate reader for your lab can be challenging. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. This protocol gives the cells viability and confluence to be monitored. FOR HTRF CERTIFIED READER. Read & analyse. HTRF Microplate Readers. Incubate overnight at RT. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within theassay linear range 6 Add 4 μL of premixed antibody solutions (vol/vol) prepared in the detection buffer. 68. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Remove the plate sealer and read on an HTRF® compatible reader . FOR HTRF CERTIFIED READER. Plates can be loaded manually or plates can be loaded using 20 or 50-plate free access magazines which fit in a stacker. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. For more information about HTRF. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. Lyse cells Read on an HTRF compatible reader . HTRF Signal + 16 µL 4 µL Standard of pre-mixed IL8 Antibodies or Sample ADD READ ANALYSE Incubate 1 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. Make sure the setup for Tb Cryptate is used. Dual monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. For more information about HTRF. 2 Locking the Plate Carrier Upon closing the software or pushing the plate carrier in/out button for more than three seconds (figure 10), the plate carrier will move into the respective lock position. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. FOR HTRF CERTIFIED READER. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. + 4 µL HTRF pre-mixed antibodies . HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. Experimental details• HTRF®-Certified Reader **. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. The plate was covered and incubated for one hour at room temperature. These plates can be read on all HTRF certified readers, thus making their implementation in. • User-upgradable microplate reader in under 2 minutes • Array of detection cartridge applications at your fingertips. Read other about this measurement approach here. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Read & analyse. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. FOR HTRF CERTIFIED READER. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. It measures 96-well plates in less than 20 seconds and 1536-well formats in under one minute. Lysates are then transferred to the assay plate for the detection of phospho-c-Src (Tyr419) by HTRF. Read & analyse. Make sure the setup for Eu3+ Cryptate is used. + 4 µL HTRF pre-mixed antibodies. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. If not, our scientific team will help you choose the instrument that best suits your needs and budget. These convert photons generated by fluorescence or. The Spark multimode reader from Tecan performs accurate and sensitive measurements of fluorescent cell-based assays. best fluorescence polarization plate reader on the market. White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. FOR HTRF CERTIFIED READER For. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The plate map included a low and a high level calibrator, conjugate blanks, and a specific control to assess the counting rate at 620 nm (i. HTRF is compatible with a large range of available microplate readers. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. At Cisbio we provide microplates that help you discover better. Learn about the principles, applications, and benefits of HTRF in this Agilent application note. • ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. CLARIOstar is equipped with a specific optical device which enables the measurement of. Conclusion. 25,000 human Jurkat cells were plated in 96 well plates. + 4 µL HTRF pre-mixed antibodies . The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. It enables you to gain insights you couldn’t achieve with detection measurements alone. For more information about HTRF. The PHERAstar ® FSX and CLARIOstar ® microplate readers offer the best possible performance for AlphaScreen ®, AlphaLISA ®, and AlphaPlex™ assays. 47 seconds for a 1536-well plate. FlexStation® 3 Multi-Mode Microplate Reader. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER culture plate to a 384-well small volume white plate. The VICTOR Nivo. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Phospho-Rb (Ser780) two-plate assay protocol. For a list of HTRF-compatible readers and set -up recommendations, please visit. Plate 25 μL of cells in. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. 96-well or 384-well small volume (SV) detection. 0. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. For more information. Introduction. LANCE ® Ultra DELFIA ® Certified HTRF Readers and Setups Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. Make sure to use the set-up for Eu Cryptate. The kit includes everything you need: reagents, buffers and a microplate. Tecan Spark multimode microplate reader, with fluorescence Fusion Optics™ meets these challenges and offers everything you need to develop and run advanced biochemical and cell-based fluorescence assays. HTRF certified . Add. Incubate overnight at RT. Lysates are ®then transferred to the assay plate for the detection of phospho- Rab10 (Thr73) by HTRF reagents. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-3 (Ser473) by HTRF. MUTANT HTT STANDARD WORKING SOLUTIONSLysis buffer 4X for two-plate assay protocol on suspension cells Determine the amount of lysis buffer needed for the experiment. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The ratio between control lysate signal / non-specific signal should be greater than 2. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-2 (Ser473) by HTRF. FOR HTRF CERTIFIED READER For. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. To perform the assay, use white plate only. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. These plates can be read on all HTRF certified readers, thus making their implementation in. EX: HTRF Emission range. FOR HTRF CERTIFIED READER . The SpectraMax i3x and SpectraMax iD5 readers can be equipped with an HTRF-certified detection cartridge (i3x) or an HTRF-certified module and filters (iD5) to meet the demanding specifications required for HTRF assays. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. These plates can be read on all HTRF certified readers, thus making their implementation in. FOR HTRF CERTIFIED READER For. BMG LABTECH’s UV-laser not only allows for faster read times in europium TRF assays, but it also greatly enhances performance in terbium-based TRF assays. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Figure 3. For more ® information about HTRF compatible readers and for set-up recommendations. The ratio of the two fluorescence Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. FOR HTRF CERTIFIED. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. Its unique optical design and instant polarizer switching provide the smallest mP standard deviation in any assay. It accepts all types of microtitration plate. Specialized filters allow for IR fluorescence. For more. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. . DLReady™ certified luminometer plate readers. Assay detection requires a microplate reader with. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYONE-PLATE ASSAY PROTOCOL: Small volume detection plate. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersFOR HTRF CERTIFIED READER. 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Read & analyse. For a list of HTRF-compatible readers and set-up recommendations, please visit . The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. at room temperature STEP 4 Remove the plate sealer and read on an HTRF ® compatible reader. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. For more information about HTRF. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. Plate 25 μL of cells in. Homogeneous Time-Resolved Fluorescence (HTRF) . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Choosing the right microplate reader ensures you’ll get an optimal readout. PHERAstar FSX Powerful and most sensitive HTS plate reader . Lysates are then transferred to the assay plate for the detection of Phospho-VASP (Ser239) by HTRF. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. It is typically used to determine the concentration of nucleic acids and proteins. For more information about HTRF. For more information about HTRF. For instance, when assay volumes are. Step 1 Step 2 Step 3 Step 4 Step 5. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. These plates can be read on all HTRF certified readers, thus making their implementation in. TO PREPARE TISSUE SAMPLES: Please refer to the Technical Note “Optimize Your HTRF® Cell Signaling Assays on Tissues” onHow to use Synergy microplate readers for HTRF detection, a versatile technology for drug discovery and life science research. -Certified Reader. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersHTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. We recommend using a white plate for time-resolved fluorescence assays and other assays where a lower signal is to be expected, to reduce the signal from background autofluorescence. PerkinElmer. Make sure the setup for Tb Cryptate is used. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READER For. Make sure the setup for Tb Cryptate is used. With its ease of use software and remote access from almost any device, it is ideal for multiuser environments. For more information about HTRF. Combined with our Kaleido™ workflow software, it. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. * For HTRF microplate recommendations, please visit . e. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Incubate 4h at RT. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. FOR READING, AN HTRF ®-CERTIFIED READER IS NEEDED. LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Better sensitivity provides better statistic, less variability among replicates, and a larger assay window. Incubate overnight at RT. • HTRF®-Certified Reader **. Plate 25 μL of cells in. Endpoint mode experiments measure each well of the microplate only once. Read & analyse. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. Cover the plate with a plate sealer. Remove the plate seal and read on an HTRF compatible reader. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. This protocol, HTS designed, allows miniaturization while. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Measure elevated and normal expression samples without multiple dilutions. Lysates are then transferred to the assay plate for the detection of total-NFkB by HTRF. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersONE-PLATE ASSAY PROTOCOL: Small volume detection plate. 96-well culture plateFor each fluorescent ligand concentration, the nonspecific binding was determined by adding a large excess of unlabeled spiperone. Advanced Phospho-ERK (Thr202/Tyr204) 2-plate assay protocol. (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based). Make sure the setup for Tb Cryptate is used. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Assay plate setup. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. 15 / Each of 1; Qty. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes• HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. Synergy H1 offers researchers a choice of monochromators or filters or both detection technologies in one compact unit. For more information about HTRF ®The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. BMG LABTECH microplate reader; Early passage Human Osteoblast cells (HOb 406-05a), supplied by the European Collection of Cell Cultures ECACC were seeded onto substrates A and B at a density of 10 4 per sample. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. It comes equipped with our patented LVF Monochromators™, filters, and spectrometer. Other technologies such as imaging. Revvity. Dual monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. VICTOR® Nivo™ multimode plate reader. Plate 25 μL of cells in. Discover our portfolio of multimode plate readers and radiometric detection solutions, complemented by assays, reagents and consumables which together enable you to increase your lab’s. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non. $1,024. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Read & analyse. 96-well culture plateFOR HTRF CERTIFIED READER. NON-SPECIFIC BINDING TOTAL BINDINGCover the plate with a plate sealer. FOR HTRF CERTIFIED READER. Incubate 2h at RT Transfer 16 µL supernatant. culture plate. A gradient of signal is apparent prior to plate reader optimization (a) causing a %CV of 9. HTRF® Europium cryptate donor / Red acceptor readout Setup recommendations for Tristar 5FOR HTRF CERTIFIED READER. It also integrates with the. As shown in Fig. Lysates are then transferred to the assay plate for the detection of total-CDK4 by HTRF. HTRF is a FRET-based technology that emerged from the Jean-Marie Lehn ’s Nobel prize-winning work on rare earth complexes. EnSight ® offers both fast well-imaging and multimode detection capabilities for target-based and phenotypic screening, all in a single benchtop instrument. Fluorescence polarization (FP) is a fluorescence-based detection method that is widely used to monitor molecular interactions in solution. VICTOR Nivo Multimode Plate Reader. Seal the plate and. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER For more. Example assays are ELISAs, DNA and protein concentration determination, steady-state dose response curves, ADP-Glo™ (Promega), Transcreener™ GDP fluorescent polarization. . FOR ORE INFORMATION Europe and other ountries 33(0)466 96 0 U. First the plate is coated with a capture antibody. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the market with all major detection technologies including Alpha and certification for HTRF® technology plus software designed for. HTRF certified . Make sure the setup for Eu 3+ Cryptate is used. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. Typical applications The HTRF cartridge provides optimal performance for key applications. It can also be further streamlined to TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate. Provide more sensitive and precise results. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed TNFα Antibodies ADD READ ANALYSE Incubate 2 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. HTRF reagent concentrations have been set for optimal assay performances. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. The multidetection microplate readers CLARIOstar, PHERAstar FS, FLUOstar Omega, and POLARstar Omega are all HTRF-certified by Cisbio, meaning each of the. FOR HTRF CERTIFIED READER. Plate 25 μL of cells in. FOR HTRF CERTIFIED READER . The controls are equivalent to the upper and lower limit of. 96-well culture plateFOR HTRF CERTIFIED READER For more information about HTRF. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The VICTOR Nivo is a HTRF certified microplate reader. FOR HTRF CERTIFIED READER. EM1 616/10 nm EM2 665/10 nm Certified for use with Cisbio’s HTRF Europium Cryptate reagents. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for• ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. + 4 µL HTRF ® pre-mixed antibo dies 2. For more ® information about HTRF compatible readers and for set-up recommendations, please. 5 EXAMPLE OF MAP PLATE . The Negative control is used to check the non-specific signal. Figure 1: Principle of HTRF Cortisol competitive assay. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). February 23, 2010, WINOOSKI VT, USA - The Synergy TM H4 Hybrid Microplate Reader from BioTek Instruments is now HTRF® certified. Incubate overnight at RT. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. The HTRF ratio obtained represents the total binding. It combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERHTRF Ratio Anti-Tag Antibody Cryptate Inhibitor-Red Inhibitor Purified Tagged Kinase Figure 2: Principle of HTRF kinase competition binding assay (IC 50 - KI determination) HTRF Dasatinib-Red Part # 62KB02REDC & 62KB02REDE Amount: 1 nmol (62KB02REDC) & 20 nmol (62KB02REDE) Concentration: 25 µM in DMSO Form:. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the. Application Area: absorbance, fluorescence, luminescence, and homogeneous time resolved fluorescence assay development. FOR HTRF CERTIFIED READER. This protocol gives the cells viability and confluence to be monitored. Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates. View Brochure. For a list of HTRF-compatible readers and set-up recommendations, please visit . Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Table 1. The HTRF cellular assays were found to be at least 16-fold more sensitive than the Western Blot. For HTRF certified readerThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. 1. Both readers demonstrated their ability to perform the cAMP G s HiRange assay with results well within Cisbio’s required. VICTOR® Nivo™ multimode plate reader. Multimode plate readers include, at the very least, some combination of absorbance (typically covering the UV/visible range), fluorescence and luminescence. -Certified Reader** *Assay volumes can be adjusted proportionally to run the assay in 96- or 1536-well microplates. The sample is then added and incubated 1. Dedicated UV-laser excitation. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation.