Spectranet customer portal. Unparalleled performance on a personalized platform. 303 unable to cal dark current. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. S. Select Start>Control panel>Device Manager. begin reading microplates with seven mouse clicks. A. SpectraMax® Microplate Readers and SoftMax® Pro Software. Shop now. Step 3: Click “Settings” and a screen will appear (Figure 1). SpectraMax MiniMax 300 imaging cytometer enables quick imaging and analysis of cells and helps in observing the viability of your cells with the field-upgradeable cellular imaging for spectramax i3/i3x multi-mode detection platform. or 0. ” So, acquisition and analysis come from one software system. Three low volume 24-well bottom slides. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. This can increase throughput and efficiency, thus saving time and space in the lab. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Single-mode Microplate Reader. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. 211, and 820 and EudraLex Volume 4 and Annex 11 and 15, and how they apply to the implementation of Molecular Devices microplate readers and SoftMax Pro. As Glazer explains, “Our readers have software that analyzes data as it’s acquired from a plate—doing both at the same time. Data analysis. The Synergy H4 is a convenient benchtop microplate reader that offers robust. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Learn moreMolecular Devices has demonstrated the value of a variety of ELISAs for a wide range of research approaches,. Supersedes 1. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. SoftMax Pro GxP Software. Products : General, FilterMax F5 reader. SpectraTest Multi-Mode Validation Plate User Guide SpectraTest Validation Plates and SoftMax Pro Software reader validation protocols. “Sensitivity is pretty much clutch for luminescence,” says Daly. The whole plate can be detect in a single run with ease and flexible. Step 1: Capture antibody binds to ELISA plate wells. Plate section without PathCheck applied. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. V. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent. The instrument settings were identical to those used in Example 1 above. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. 425 or. 2. Transfected cells in the 6-well plate were trypsinized, divided into ten aliquots per well, pelleted at 1500 rpm for 5 minutes, and washed once with PBS. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. Or, with the reader specifications noted from the Instrument. Microplate Readers. The plate reader. #0200-6263) UltraVision™ Plate 384 (Brooks Life Sciences cat. Published Dec 16, 2020 | Updated Dec 16, 2020. Compact designs. Step 2. VIS absorbance detection. Instrument settings can be saved as a protocol file. 2. SpectraMax iD3. Search. Turn on Plate Reader and wait for it to complete its start-up routine. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. 2. ~ Bio-Tek ELx808 Microplate Reader $2,795. The procedures for these tests are outlined in the instrument user manual. When connecting for the first time to either the SpectraMax L reader or StakMax system. 635. Category 2 and 3 microplate readers use gradient heating as well. #P7589 or P11496) • Black 96-well plate (Greiner Bio-One, cat. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. Tests every subsystem of the reader . The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Table 1: FLIPR hardware settings for a cell plate basal fluorescence signal test. These systems are hybrids that allow you to choose between. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. Built-in Help function answers your most frequently asked questions. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. For more sample throughput, the system can be easily integrated into a full roboticThe SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. These models can no longer be repaired. Ratios for each sample were calculated as Read B/Read A. The reader measures both flat and round 96-well microplates and. A. 6 % of reading 4. #Flex3) FlexStation 3 8-channel Pipettor Head (Molecular Devices cat. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. coli stock with an OD 600 value of 0. In . Exploring misconceptions and understanding the technologies. Plate stacker and robot integartion. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. e. The touchscreen interface provides integrated instrument control, data display, and the ability to export results for statistical data analysis. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for. 1. Recertification service. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. HTRF-compatible SpectraMax Multi-Mode Readers. System setup and calibration are controlled from within SoftMax Pro Software. Testing can be done in the user’s lab on their own schedule. Endpoint. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. 190. Absorbance microplate readers from Molecular Devices are used to detect the absorbance in the melamine assay. SoftMax Pro Software. Advantages of multi-mode plate reader technology A multi-mode reader has many advantages for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation to cell imaging. Step 3. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. absorbance plate readers. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Installation Qualification (IQ)the iD5 reader’s filter system. 020 OD 2. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. Molecular Devices' microplate readers. Turn the reader off by pulling the power cord. View the Molecular Devices website. Supersedes 1. Page 1 of 8 . Next generation SpectraMax® absorbance microplate readers. Eliminate time-dependent substrate addition steps. the use of a reader calibrated with primary NIST and NMI standards. Molecular Devices 推出了FlexStation® 3 多功能读板机系统。FlexStation 3 把 SpectraMax M5e 和 8 道 / 16 道的移液 系统整合在一起成为体积小巧功能强大的 读板机系统。该整合系统不但为用户提供 了多功能检测平台，还增加了液体处理的 通量和提升了检测生化和细胞动. Feb 22, 2023Fast and compact absorbance microplate readers for a wide range of assays without the use of filtersSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Connecting Instrument Cables The power cord and USB cable connect to the ports on the rear of the instrument. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. ¨Automated microplate readers reduce manual manipulation and ensure consistent operation. 9. 00. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Avoid losing high signal. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemPrimary considerations when choosing a microplate reader are throughput and flexibility. That’s why Molecular Devices offers the following services: Installation qualification (IQ), operational qualification (OQ), preventive maintenance (PM), and repair coverage. Read times. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. ** Some compatible filter sets are available for purchase from Life. SAN JOSE, Calif. 32 was diluted 10-,. SpectraMax Paradigm Multi-Mode Safety Guide From plate readers to high-content imagers, Molecular Devices has the instruments you need to advance your discovery. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. (a) Chemical structures of 1 (HADP) and its analogues 2–4. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. The user guide also covers the software features, the maintenance procedures, and the technical specifications of the readers. Multimode Microplate Readers. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with all This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Expanded wavelength coverageAre you looking for a versatile and affordable microplate reader that can perform various assays and measurements? Check out the FilterMax F3 and F5 Multi-Mode Microplate Readers, which offer absorbance, fluorescence, luminescence, and more read modes with high performance and reliability. were started immediately with a Molecular Devices FlexStation Plate Reader. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. The plate was placed on a shaker for 30 seconds and then incubated for 3 hours at 37°C, 5% CO 2. Next generation SpectraMax® absorbance microplate readers. View SoftMax Pro GxP. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). We would like to show you a description here but the site won’t allow us. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. the standard plate reader applications, protecting your initial investment. ELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. Read More. absorbance readers. Learn more about how absorbance is measured, and some key applications that utilize absorbance. Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. SoftMax Pro Software. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. Contact Us Request Quote Microplate Reader Comparison Charts Published Dec 16, 2020 | Updated Dec 16, 2020 Products : General, FilterMax F5 reader Please. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. ABS Plus can run both cuvette-based and microplate reader applications on the same instrument. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. 1 up to 3600 sec. Remove the lamp screw (on the right side of the lamp, as shown in the diagram) then gently pull the connecting wires to remove the lamp from the body of the instrument. 9000-0756. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm) with 4 filter choices included at time of purchase. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into cardiomyocytes and neurons, up to 100 times per. 0112-0127C 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 9 ComputerIntegration 11 InstrumentCovers 11 FluidicsModule 12Plate reader instructions Computer. Also refer to the Multimode Reader Comparison Chart Features. We would like to show you a description here but the site won’t allow us. M5. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Fortunately, Molecular Devices’ SpectraMax i3x and iD5 readers offer the best of both worlds. AXON Patch-Clamp. 1. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. The sample is typically placed in a cuvette through which light is sent horizontally. The SpectraMax 190 reader can be integrated with Molecular . Perkin Elmer Victor X2 Multimode Microplate Plate Reader 2030-0020 w/ PC. The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. Plus 384. The adapter is required for optimum performa nce with standard 96-well and 384-. SpectraMax® Microplate Reader Suite of Products Knowledge Base. FlexStation 3 Multimode Microplate Reader (Molecular Devices Cat. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Eight filter options provide simplified assay setup. (b) Fluorescence responses of probe HADP and control compounds. Plate Formats: 6 to 384-well microplates. together with a Molecular Devices plate. : 8 Oct 12 Page 1 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0140 Rev. Absorbance Plate Reader Comparison Chart. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 18 kg. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. If the computer is connected to the Internet: Select Search Automatically for. The optical system uses two scanning monochromators so you can determine optimal excitation and emission settings, resulting in. #6590-0118) Filter 665/10 (Molecular Devices cat. 1. Microplate Readers. Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. Luminescence was read every five minutes for five hours with three seconds of orbital shaking before each read. 2. 76358-624. The assay is easy to set up and the range of potential analytes is vast, but the assay procedure is time consuming and labor intensive. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. 1700 Online: Sales Offices USA/Canada 800. 9. 9. Page 16 FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide To exchange an excitation or emission filter in a slide: 1. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. FlexStation™ II Systems provide integrated fluid transfer capability from a source platePlate Reader SpectraMax iD5 multi-mode system Plate Washer AquaMax 4000 Microplate Washer Robot PreciseFlex 400 Sample Handler Plate Storage Plate Hotels – 40 microtiter plates Controlling Software Genera Control Software Incubation LiCONiC STX44 Automated CO 2 Incubator Liquid Handler Microlab STAR Automated Liquid Handling The. 1. 2 GxP Software is our most secure software to help you achieve full FDA 21 CFR Part 11 compliance with streamlined workflows to ensure data integrity. What are the physical and environmental operating specifications for SpectraMax UV/Vis and Gemini plate readers? (With extra info on backup power UPS choices). Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. and California)Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. Select Start>Control panel>Device Manager. The FlexStation 3 reader exclusively offers fluid transfer validation using Molecular Devices patented PathCheck® Feature to quantify the integrated 8- and 16-channel pipettor head performance. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). An industry-renowned instrument for monitoring GPCRs and ion channels. It consists of a SpectraMax® Microplate Reader, Peak Robotics S-LAB Plate Handler with the S-LAB software, and the AquaMax® 4000 Microplate Washer. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and software solution for . Microplate readers from Molecular Devices are designed to provide consistent performance for many years. For more sample throughput, the system can be easily integrated into a full robotic The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. At the beginning of a data run, perform a ìsignal testî to check the basal fluorescence signal (i. This document outlines references to 21 CFR Parts 58, 211 and 820 and EudraLex Annex 15 to assess the implementation of Molecular Devices microplate readers in regulated environments. A standard spectrophotometer measures the absorbance of one sample at a time. An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. #iD3) Methods. PURPOSE. Skip to main content 美谷分子仪器（上海）有限公司 咨询服务热线：400-821-3787. FAST AND ACCURATE METERTECH Microplate Reader $3,650. D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Plate pre-read measurements were made in a Costar UV plate. The CellTiter-Glo assay from Promega uses luciferase. Step 2: Launch SoftMax Pro Software. The adapter is required for optimum performa nce with standard 96-well and 384-. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. 304 signal level saturation. FlexIII. The primary mode is fluorescence. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Accurate and sensitive detection of nucleic acids and proteins are critical to many experiments. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Microplates for Molecular Devices Microplate Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. 0200-6262. Step 4: Add detection antibody – Enzyme-conjugated detection antibody binds to a second site on the. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax®. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Multi-mode Microplate Readers. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Microplate Reader, Multimode Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader w/ SoftMax Pro. 100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. PURPOSE. SoftMax® Pro 7. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. The former have been the traditional domain of plate readers and great strides have been. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. Published Dec 16, 2020 | Updated Dec 16, 2020. Learn how you can utilize microplate readers to drive landmark discoveries. Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. SpectraTest ABS2 Absorbance Validation Plate SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e,. Get the most out of SoftMax Pro. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. for SoftMax Pro Software. 997 (n = 3). Turn on Plate Reader and wait for it to complete its start-up routine. Supplier: Molecular Devices. W×D×H. Shop now. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. One plate carrier adapter is provided with the instrument. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. We would like to show you a description here but the site won’t allow us. Cell line development teams will realize savings on time and resources,SpectraMax i3x. 5 sec. The Gemini instruments can read some luminescence but it is about 100 times less sensitive than a dedicated luminometer. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). 0) OD (500 to 1100) RFU (5. SpectraMax® Microplate Readers and SoftMax® Pro Software. visible absorbance, fluorescence. View SoftMax Pro GxP. For biochemical assays, the washer removes unbound material and unreacted reagents. Other features and benefits include: Eliminate changing out filters. 0. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. Contains a revised proprietary masking dye with higher light extinction in the extracellular solution to significantly reduce background and increase signal window. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Chapter 1: Introduction The SpectraMax® iD3 Multi-Mode Microplate Reader from Molecular Devices is a monochromator-based, multi-mode plate reader. . White plates reflect the light, enabling even low levels of Luminescence to be detected. EUROPE. Users of Molecular Devices' SpectraMax® i3 Multi-mode reader can even add an optional. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Fig 2: Enzymatic response of synthetic compounds. The low profile increases robotic stack capacity and storage. On the back, in the right hand top corner, there. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. Do you want to learn how to use the FilterMax F3 and F5 Multi-Mode Microplate Readers for your research? Download the user guide and find out how to install, operate, and troubleshoot the instruments. Page 1 of 8 . Flexibility to read a variety of plate formats. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Microplates for applications that require high sample recovery rates. plate readers. Select Update Driver. ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. Flip open the carriage door and using a flash light to check where the plate is stuck and whether it has been damaged. With three modes of detection for full spectrum absorbance, fluorescence and luminescence, the SpectraMax® Mini reader . • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. Read one sample or up to. The plate reader can measure multiple readouts, including UV and. 5577 UK +44. Select the Driver tab and click Update Driver. This can increase throughput and efficiency, thus saving time and space in the lab. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Test up to 3. 00. 314 low lamp level warning. Single- and multimode microplate readers are useful for very broad range of applications including ELISA, kinetics and high throughput screening assays. This page contains the. Molecular Devices provides the complete solution covering the instrument and software: • SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes • IQ/OQ for all microplate readersSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Highly customizable low volume applications Molecular Devices unique SpectraDrop™ Micro-The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it.