384-well microplate compatible Well read area range: H7 to K22 Figure 1-3 24-Well Micro-Volume Sample Slide Figure 1-4. Plate formats. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. It can be performed using any well in the plate with a relatively strong fluorescent signal (i. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 00. *For Corning UV plates, mean values at 260 nm ranged from 0. Both the P and M injectors were set to deliver 100 µL (96-well plate) or 25 µL (384-well plate), with a 2-second delay and 10-second integration time. x. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. Devices PathCheck® Sensor—the only patented technologySpectraMax ® M5 Multi-Mode Microplate Reader (Molecular Devices) Gemini EM Microplate Reader (Molecular Devices). The equation that allows one to calculate absorbance from % transmittance is. Four wash/rinse bottles are included and configurable for both 96- and 384. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. Note: These settings are based on necessary cookies. Others models hold the sample in place using surface tension. The SpectraMax Paradigm Platform was able to quantify the protein down to the low picomolar level using an AlphaLISA method (Figure 1). 96- and 384-well microplates. More Product Information. These lab workhorses are used in drug discovery, research, bioassay validation, and. readers are the industry’s most cited instruments and have empowered life science researchers to advance protein and cell biology, breaking the barriers to novel, landmark discoveries. Time-resolved phosphorescence. Typical 4 min/ 384 2 min/384 8 min/1536 Throughput plate plate plate Fully enabled protocols = reduced setup time. B. Minimum volume and maximum throughput with 96- or. M5. Turn the. Microplate readers provide the ideal measurement platform for the high-throughput. 01% BSA starting at a concentration of 1 x 10 6 fg/well was prepared, and 25 μL of each concentration was added in triplicate to a 384-well solid white plate. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. 2. Values are averages from 5 clean plates, each filled with 200 µL water/well. Plate reader instructions Computer. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. 4 Equipment. The calculated LOQ values were 0. 96/384-well plate formats. BioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. Dimensions: 18. 046 to 0. x. Versa Max. SpectraMax® Plus 384 provides ultrafast, full spectral range. 8 mm distance between well bottoms to plate bottom. 12, 24, 96 & 384-well SBS microplates; Range of 96, 384-well SBS microplates . After incubation, the assay plate is transferred to the seal-peeler to remove the seal from the assay plate and transferred to washing. 314 low lamp level warning. Outer wells are aligned for proper viewing with FLIPR. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. 384-Deep Square Well Plates. 384-well TC plate, Poly-d-Lysine, Flat Clear. x. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. FlexIII. 96-well fluorescent cell dilution series, top-read. Alpha 384 HTS 96- and 384-well . (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. Important things to consider when purchasing a multimode microplate reader. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the. x. SLS & DLS: 2 µL quartz cuvettes 45 µL quartz cuvettes 4 mL generic glass cuvette DLS only:Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of. 00. (or plate reader). #2704174, 2705402, 2705412, 2704180; Drummond Digital Microdispenser cat. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. Ideal for miniaturized 384 well fluorescent. 100 µL (96-well assay) or 25 µL (384-well assay) of CellTiter-Glo reagent was added to each well. After reading the ELISA plate on both readers, each standard curve was nearly identical. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Fluorescent reading data were analyzed by non-linear fitting (GraphPad Prism, GraphPad Software Inc. 5 ng per well. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. Contact our support team with your questions regarding Molecular Devices products and applications. x. Note: if all wells of a 384-well plate are to be read, the kinetic interval may need to be. > Molecular Devices LLC > 384 well plates. (1536-well plate) 2 µL (quartz cuvette), 4 µL (disposable cuvette). The Embla 96/384 Well Washer accepts 96-and 384-well format microplates and is ideal. . Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. Also refer to this Multimode Reader Comparison. and the A1 corner of the plate adapter (Figure 1-2). Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. Learn more about SpectraMax Plus 384 Microplate Reader >>Rating: 5. Molecular Devices Gemini XS Microplate Spectrofluorometer Details. 384 Well Optically Clear Tissue Culture Plate: Plate length: 127. #3635) Transferpette micropipette (Drummond Scientific cat. Figure 2. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Two high-energy xenon flash lamps: Reading capabilities. 8mm distance between well bottoms to plate bottom 50 µL total well volume, with recommended working volume of 5-40 µL High base plate design is compatible with automation and for bar coding Validated by Molecular Devices for the FLIPR reader. Cell viability는 세포막 Integrity 또는. These robust readers have been placed in labs from Antarctica to the. The price of a microplate reader depends on the technical equipment and the number of measurement technologies that the device can measure. 1: The SPECTRAmax 340PC 384 System The on-board microprocessor calculates and reports the absorbance (or % Transmittance) for each well of a microplate. x. In addition, SoftMax Pro SoftwareSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. a list of plates specifically supported shows, 96, 384, 24, 12, 6 well types. All microplate washers enable users to program tank selection, volumes, step times, and cycles for customized procedures. begin reading microplates with seven mouse clicks. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. Next-generation luminescence plate reader. Two different agonists, acetylcholine and carbachol, were tested. x. 00. Growth medium was then replaced with fatty acid uptake solution. 0 (or higher) Software; Methods Data acquisition using the Workflow Editor. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. We would like to show you a description here but the site won’t allow us. 6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. SpectraDrop High-Throughput Screening (HTS) Kit. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Visible absorbance -. Molecular Devices, LLC. 19 H mm that meet microplate industry standards. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modes <2 fg/well for firefly luciferase in 96- and 384- well top read. 96-, 384-, and 1536-well microplates. For Research Use Only. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. This application is highlighted by the example of a fully automated kinetic solubility screen in 384-well plate format. Catalog number: BC1384W. and 25 μL of each concentration was added in triplicate to a 384-well solid white plate. 6-well tissue-culture treated (TC) plate, sterile. To address this concern, Molecular Devices offers the FlexStation® 3 Multi-Mode Microplate Reader. Some instruments can handle only 96-well plates and lower, whereas others can read up to the ultrahigh throughput densities of 1,536-well plates. 96 deep well plate: packed in sleeves of 6 , 24 per case. The Standard Thermo Scientific 384-well plate, Barcoded, White, is compatible with the majority of 384 block PCR and sequencing instruments. Optional. …200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. 76 ± 0. Page 1 of 8 . x. X. ability to use as little as 2˜µL samples with 24- or 64-well plates. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. 6-384 (96- and 384-well with pipetting) 96-well microplates. For more sample throughput, the system canSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. 3% in white 96- and 384-well microplates < 3 x 10-5 (white plates) < 3 x. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Ensure data accuracy of your reader. Important things to consider when purchasing a multimode microplate reader. Align the two slots on the bottle holder with the two pegs on the rail. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. Plate chamber design of the SpectraMax category 1 microplate readers. Embla washers are durable and easy to maintain. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Wash heads are made of Polyethylene Terephthlate (PET) with stainless steel probes to prevent corrosion. B. M2. Select Consumables. 384-well SBS microplates are designed with corners cut at a1 and h1 positions to conform to SBS nomenclature. It is more inert and reduces compound interference. x. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. The FilterMax F3 can read 96-well and 384-well plates. SpectraMax® M2 and M2e Microplate Readers are the standard for UV/visible multimode reader absorbance, providing ultrafast and full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 5 ml microcentrifuge tubes 35 mm Petri dishes 12 mm × 50 mm test tubes (optional). Round well design with a solid black flat bottom2. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. Selects either 96-well or 384-well plate for a stand-alone read. 00. Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. Visit the supplier page or submit a quote to learn more. 0 and dispense 10 μL to all wells of the 384-well plate(s) (see Note 13). Guaranteed sensitivity: < 100 amol of biotinylated-LCK-P peptide, 25µL/well in a 384-well plate. Reconstitution of reagents. highthroughput alphascreen, alphascreen on plate reader Created Date:SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. The color touch screen provides a visual user interface, making programming fast and intuitive. x. Use standard plate unless you see your type listed. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. #2704174, 2705402, 2705412, 2704180; Drummond Digital Microdispenser cat. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. Outer wells aligned for proper viewing with FLIPRIdeal for miniaturized 384 well fluorescent homogeneous assaysNot treated (or medium binding)… Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Reading Capabilities: cuvette or microplate. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. 96/384-well plate formats. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. It has a. This is equivalent to having 511 built-in filters, 384-well: 0. x. x. FLIPR Calcium 4 Assay Kit (Molecular Devices, San Jose, CA, USA). The system provides automation for up to 50 microplates for easy walkaway automation. No accessories are needed and SoftMax® Pro Software. 0200-6262. With the FLIPR 384 , you get the sensitivity, data fidelity, and real-time, kinetic information of the FLIPR I system in a 384-well mode. efficiency gratings, well scanning up to a 20x20 read matrix, spectral scanning and detection of plate formats from 6 to 384 wells, the SpectraMax iD3 reader is the complete solution. Data for a 384-well plate can be collected in less than two minutes, as opposed to up to 30Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. Outer wells aligned for proper viewing with FLIPRIdeal for miniaturized 384 well fluorescent homogeneous assaysNot treated (or medium binding)…Elisa Plate Readers and Microplate Readers at Biocompare. • Tyrosine Kinase Plate Please contact your Molecular Devices sales representative. Recertification service. SpectraMax M2 and M2e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 384-Well Microarray/High Sample Recovery Plate. Formats available are strip washers, full plate washers and combination washer-dispensers. 2. #3-000-510)Page 154: Ordering Parts And Accessories. All measurements were performed in six replicates and cell toxicity was calculated as the percentage of the OD value of untreated or treated. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Add 10 µL of ADP Detection Mix to those rows. 1 pM 1536-well: 0. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. smartinject technology, atp a, spectramax i3x multi-mode microplate reader, luminescent cell vi, spectramax i3x multi mode microplate reader, spectramax i3x. ©2022. 96-, 384-, and 1536. The FLIPR Fluorometric Imaging Plate Reader system addresses the industry's requirements for higher throughput, minimized sample volume, and cost reduction. assay volume in a 384-well plate 0200-7017POS Alpha 384 STD 96 and 384-well plates < 100 amol (384-well) 1 0200-7018POS Alpha 384 HTS 96 and 384-well plates < 100 amol (384-well) 1 0200-7019POS Alpha 1536 HTS 96, 384, and 1536-well plates < 100 amol (384-well) 1 HTRF Cisbio HTRF detection with optimized Xenon light source and 616, 665 nm EM filters The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Page 154: Ordering Parts And Accessories. For more sample throughput, the system canThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. ‘StimB’ is Stimulation Buffer, included in the IP-One Gq kit. Our microplate. Documentation:Molecular Devices microplate readers. That explains the lower values on the LmaxII384 plate reader. 2. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. The VICTOR Nivo. , April 14, 2022 – Molecular Devices, LLC. x. With optimized reagents, option to operate the. Abs). com 上海 电话: 86-21-3372 1088 传真: 86-21-3372. 48 W x 12. Molecular Devices SpectraMax Plus 384 Absorbance Reader Details. FLIPR Calcium 4 Assay Kit (Molecular Devices, San Jose, CA, USA). The screw remains inside the instrument. the Molecular Devices SPECTRAmax® 190 and 250 plate. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. Browse for different fluorescent readers below and compare across different instrument suppliers. . The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 4 decades . Industry standard disposable (choose from recommended list). 7. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. 313 reference gain check fail. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. The analyzed results are. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. F3/F5 Multi-Mode Microplate ReadersBoth reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Outer wells aligned for proper viewing with FLIPR; Ideal for miniaturized 384 well fluorescent. Packed 25 plates per sleeve, 2 sleeves per case. The systems can handle any slide-based microarray study, from small one- or two-fluor applications to multiple-fluor high-throughput projects requiring automated. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . Molecular Devices has developed the FLIPR Membrane Potential Assay Kits to maximize. For more sample throughput, the system canThe SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Microplate format. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Use the provided 3. Method 1. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Products : Ionworks HT, Ionworks Quattro. 150 µL of deionized water was pipetted into each well of a clean 96-well plate. Industry standard disposable (choose from recommended list). For more sample throughput, both readers can be easily. Assay setup for a 384-well low-volume plate. With optimized reagents, option to operate the readers. Scan every well . The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. VMax . Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Get Quote . With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. In simplest terms, a plate reader measures and. 7. x. Following the flushing of the machine as described in Section 3. For biochemical assays, the washer removes unbound material and unreacted reagents. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax Plus 384 Microplate Reader (Molecular Devices cat. All other trademarks herein are the property of Molecular Devices, Inc. MultiWash™+ Microplate Washer benefits include, walk-up usability, 4 wash and rinse. Experience the identifiably different. SpectraMax® iD5 Multi-Mode Microplate Readers,6 to 384-Well Microplates, Molecular Devices, LLC (CAT#: STEM-LE-0504-LC) Product Keyword. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. 2. VIS absorbance detection. Molecular Devices products are warranted to meet the stated specifications. The results above revealed excellent detection limits and dynamic range in all three Molecular Devices microplate luminometers. Measure aggregation, aggregate size distributions, changes in solution molecular weight, and propensity for aggregation: indicators of both thermal stability (T onset, T m, T agg) and colloidal stability (A 2 /B 22,. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. Each kit is sufficient for 3 Calcium 6 assay plates and 3 Calcium 6-QF assay plates. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Backed by the. Unparalleled performance on a personalized platform. SpectraMax i3x. Total volume (except deep. Comprehensive solution. 190. #S1205. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. 5577. The SpectraMax Paradigm reader with HTRF Detection Cartridge offers high-throughput screening capability with excellent read times and Z’ factors. x. The eight-channel system delivers both high precision and speed when reading 96- and 384-well microplates for endpoint measurements, kinetic assays, and spectral scans. x. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Step 1: Open the SoftMax Pro IMAP FP protocol. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. plates < 100 amol (384-well) 1 0200-7018. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Outer wells aligned for proper viewing with FLIPR Ideal. 3. Read one sample or up to 384 samples in a single plate by using any standard cuvette, or 96- or 384-well microplate. Outer wells aligned for proper viewing with FLIPR. SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. #0200-7011) Instrument setupRound well design with a solid black flat bottom2. #0200-7011) Instrument setup The results above revealed excellent detection limits and dynamic range in all three Molecular Devices microplate luminometers. M5. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. 2 OD at 200 nm; this decreased to 0. Total volume (except deep well plates) is 120 mL. Artel MVS Bundle DM Std/800TSNB Reader Details. Fluorescence Microplate Reader Comparison Chart. [Carbachol] (nM) RFU Max. Results Microplate readers read biological, chemical or physical events that occur on a microtiter plate. SpectraMax ABS Plus reader utilizes Molecular Devices PathCheck Sensor- the only patented. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. $542. 8 mm distance between well bottoms to plate bottomValidated by Molecular Devices for the FLIPR reader. A snapshot of the Settings screen is shown in Figure 2. for ELISAs and most cell-based assays. Temperature control: 4°C above ambient to 45°C. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. The all new CloneSelect Imager FL features high contrast multichannel fluorescent and white light imaging that allows for accurate single cell detection and proof of monoclonality at day 0. Shaking: Linear, orbital, and double orbital. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. (40 µl maximum) Clear, white, or black. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. X. Plus 384. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. Instrument pricing. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. Two high-energy xenon flash lamps: Reading capabilities. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells. plates < 100 amol (384-well) 1 0200-7019. Microplate Formats: 6, 12, 24, 48, 96, 384. Microplate readers from Tecan; cutting edge bench-top microplate readers lead the industry in both versatility and performance. Unmatched throughput FLIPR Membrane Potential Assay Kits allow the elimination of wash steps and much shorter read times on the instrument. Each kit is sufficient. This can increase throughput and efficiency, thus saving time and space in the lab. x. We would like to show you a description here but the site won’t allow us. ©2013 Molecular Devices, LLC • 6/13 • Printed in USA • PN: 0120-1523. Dimensions 128 x 86 x 15 mm.