Please click on the links below to view the Microplate Reader Comparison Tables. In addition to increased throughput, microplate formats enable researchers to miniaturize their analysis (using less sample and reagent) to study regulatory events and. 0025 OD from 0. Reaction formats The MagMAX™-96 DNA Multi-Sample Kit can accommodate a broad range of throughputs. d. 90349). UV cuvettes are ideally suited for measurements at 260 nm, 280 nm and in the visible range. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. x. 0001 ABS (calculated) • Light source: Halogen lamp, 8V/50W • Wavelength range: 405, 450, 492, 630 nm, 4 more filters optional. High-throughput, automated dynamic light scattering. Packed 25 plates per…. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Never order or replace filters to optimize an assay again!6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. Bowman Dispensers. $3,207. The absorbance was then read on the SpectraMax microplate reader at 426 nm, and the resulting OD values for all wells were averaged together to obtain a value of 0. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. Learn more. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 01. of 3 wells of a 96-well plate, from 1 of 3 independent experiments. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. 1 OD at 230 nm. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. Fluorescence Microplate Reader Comparison Chart. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Uncontrolled Copy for Training and Reference Purposes Only . Working in. M5. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit. A = 2 - log 10 (%T). Page 1 of 8 . From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. With more than 40 years of plate reader expertise and life science research, we’ve amassed an extensive. President of Molecular Devices. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Tecan’s quality control and validation packages help our customers to fulfill all regulatory requirements for their Tecan microplate readers in an efficient, cost-effective way. SUPPORTED PLATES Microplates having 6, 12, 24, 48, 96, and. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. Our. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm: Measurement Type: Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra: No. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. EMax. The device was able to achieve 3σ limits of detection of ca. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. 7°C (gradient heating design). 0. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. 2 OD at 200 nm; this decreased to 0. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Unparalleled performance on a personalized platform. This is equivalent to having 511 built-in filters,The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. Microplate readers compatible with 96-well plates Single- to multi-mode readers combine an absorbance plate reader , a luminescence plate reader , and a fluorescence plate. Kinetic assays were read every 20-28 seconds for one hour,The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 0% Normal and Rapid modes, 96- and 384-well plates; Absorbance Repeatability: 0. 2% in. 00. The CellTiter-Glo assay was used to measure ATP. #C1500) Control standard endotoxin (ACC cat. What is the dead volume in 96 and 384 well compound plates? Dead volume is 5 micro liters. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. Figure 2-23: Bottle holder To install the bottle holder: 1. 000 – 4. Supersedes 1. UT-6550 Microplate Reader is a reliable and robust instrument for a wide. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. 5°C at 37°C. 20 plates/hour (2-minute read time) 96-well 1,920 data points/hour 384-well 7,680 data points/hour 1536-well 30,720 data points/hourMicroplate Reader, For Lab. adjust the reader. Selects either 96-well or 384-well plate for a stand-alone read. Some will only support one well density (e. The EMax Endpoint ELISA Microplate Reader from Molecular Devices (one of the pioneers of microplate detection) provides a simple and durable dedicated endpoint detection method. With theMolecular DevicesWe designed and fabricated different sized microfluidic covers, matched to the desired experimental conditions: Fig. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Read up to 2 wavelengths in endpoint absorbance for 96-well plates. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Top and bottom reads are. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. x. Dimensions: 18. Determine concentration using the Beer-Lambert LawPage 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. Selects either 96-well or 384-well plate for a stand-alone read. Instrument settings can. Detection of KASP genotyping reactions. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. 2% in. More Product Information. 304 signal level saturation. 0% ± 0. Temperature control range from Ambient +4º to 50ºC. Through absorbance readings from both the ultraviolet and visible spectrum range, it can determine the concentration of samples, conduct positive or negative detection of antibodies and. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. 0% ± 0. 057, depending on lot number. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. BMG Labtech - THERMOstar. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. 000 to 2. Figure 1. On the day of the assay, the plate was equilibrated. Back plate and…. Step 2: Launch SoftMax Pro Software. Page 1 of 8 . Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. The screen on the left shows the wells selected for Method MI AF488-10 and Method MI AF488-5 for image acquisition from rows A-D of a 96-well plate at. The plate was preincubated in the reader at 37°C for a minimum of 10 minutes. 00. 20 µL (96-well plates) or 10 µL (384. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. Dark blue indicates well will be read. Molecular Devices LLC Applications: Inquire; Plate Formats: 96-, 384- well. 9000-0756. 0. 340 PC384. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. They can be read by most microplate readers. QuickSync. Kinetic readings were started immediately with a Molecular Devices FlexStation Plate Reader. Range of 96, 384-well SBS microplates . Temperature control: 4°C above ambient to 45°C. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. one 96-well sample plate can be controlled from 15–40°C,. 1 x plates; Instrument dimensions. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. Spectramax ® Plus microtiter plate reader (Molecular Devices, Sunnyvale, CA) or similar UV-Vis plate reader; SoftMax ® Pro software (Molecular Devices) (optional) UV compatible quartz plate (for example: # R8024; Molecular Devices) or 96-well Costar ® with UV transparent bottom (for example: #3635 ; Corning Inc. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in. Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm. Top and bottom reads are. Microplates, also known as microtiter plates, are rectangular multi-well plates used in a variety of assays from ELISA to PCR. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. LogPhase 600 is controlled with a software App that provides a simple, yet powerful, user experience. 1. Published Dec 16, 2020 | Updated Dec 16,. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A) Flexibility to read a variety of plate formats. Part Number. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. These microplates have a corner notch for use in standard plate readers. These instruments deliver high precision and speed when reading 96 Well Microplates for single endpoint measurements and kinetic assay data gathered over time. Absorbance cuvette port. The Phosentix MicroQC™ Plate is an advanced microplate reader analysis tool that offers exceptional multi-modal functionality. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Washers configured for both 96- and 384-well plates. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . #3693; n = 4 for each standard). . Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Inconsistent results: cannot determine whole well confluence – well after well. • High antibody-binding surface improves the overall performance in. The plate reader can measure multiple readouts, including UV and. 96- or 384-well microplate compatibility. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. To help you get the best results from your Molecular Devices® plate readers it is essential that you choose the right plate. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty EarlyTox Live/Dead Assay Kit (Molecular Devices cat. Dynamic range > 6 decades . BioTek LogPhase 600 microbiology reader measures microbial growth curves in up to four standard 96-well microplates at a time. The soil was amended with either 1. % transmittance of plates used in a plate reader. The system can perform readings. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. An industry-renowned instrument for monitoring GPCRs and ion channels. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. The 8-channel read head allows for fast read times which means a 96-well plate can be read in as little as five seconds. #23225). Luminescence applications and assays. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of the human AT1R receptor. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. 1. 46Click the Read button in the SoftMax Pro3 reader combines Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact. XTR Rack Pro 96 (Vial Rack Bottom Label 2D Reader) BIO-LAYER INTERFEROMETRY MICROPLATE READING: Forte Bio: Octet HTX: Octet QK384:. Elevated well walls for easier sealing Round bottom formats Square and round well formats Conform to SBS standards Lids and cap mats available Packed in sleeves of 6, 24. Compact designs make these instruments. The variations include adjustable. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. 312 gain calibration failed. 96 well plate injection time with SpectraMax® L plate reader. 00 USD. Figure 1-2 Match chamfered corners of slide and adapter 2. This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. A polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. flat-bottom: 50-60 µl. The IC50s by cell rounding and LysoTracker. Wavelength(s):. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. Products Clone Screening Accessories and Consumables Deep-well Plates Deep-well Plates Deep well blocks available in 24, 96 and 384-well formats Product Options. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Microplate Spectrophotometers. #CA961) Methods. Your applications, your modes, your choice. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. During preincubation, the working reagent was prepared by mixing fluorogenic substrate, assay buffer, and rFC enzyme solution in a 5:4:1 ratio, respectively. Compare this item. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. FlexIII. HeLa. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. 1 – 2. Eight filters enable visible light absorption measurement applications such as protein quantification, cell viability and ELISA. D. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. Create custom fluorescence assays without having to purchase expensive filter sets. NanoQuant plate. • Gently wash cells once with 200 μl (for 96 well plate) or 400 μl (for a 24 well plate) warm (37°C) 1X DPBS (containing Mg and CaCl 2). 00. . Align the two slots on the bottle holder with the two pegs on the rail. FlexIII. Capabilities. MCF7 cells treated with. No. 993 (Figure 3), validating the assay’s usefulness over a wide range of viable cell numbers. Site. Find our technical support information along with contact details. #CA961) Methods. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. tools to expand the boundaries of their research. SoftMax Pro GxP Software version 5. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Other. Features of Clear 96-Well Microplates: • Clear plates for colorimetric ELISA and other assays. Published Dec 16, 2020 | Updated Dec 16, 2020. #ID5-STD) Pierce BCA Protein Assay Kit (ThermoFisher Scientific cat. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Washer maintenance kit: 2 X T 2. Download a PDF version of the SpectraTest validation package User Guide and the ABS2 SpectraTest validation protocols. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. The SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . Including injection time, it takes approximately 68 secs. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Absorbance equation. 5 OD at 492 nm Computer interface USB 2. It offers extremely low absorbance background, excellent. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Infinite possibilities. Linear, Orbital Slow, and Orbital Fast. A barcode reader accessory can be purchased with theWe would like to show you a description here but the site won’t allow us. Users can choose to process all plates within the magazine or select a specific number. com Molecular Devices (Germany) GmbH ismarcring ierach an der iss erman ©2019 Molecular Devices, LLC. )Plate dimensions: X6001, X6004, X6011, X7001, X7007, X7020, X7022, X5101, X6026. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. x. * Note: Programming the washer with a final aspirate height of 4. 50/plate. 96-well) while others have flexibility to. Description. 0, or. Temperature control: 4°C above ambient to 45°C. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Two different agonists, acetylcholine and carbachol, were tested. variety of. For the kinetic and endpoint LAL assays, the ELx808 TM Reader is highly reliable and easy to use. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Plate. External Dimensions without lid (mm). x. #C1500) Control standard endotoxin (ACC cat. An example of dual-luciferase assay results. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for. Suggested plates are black walled. 5 pM Luminescence performance Wavelength range 300–850 nm Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1. Self-righting base, up to 40 degrees past center without dispenser. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Cells were transfected as previously described and plated at 30,000 cells per well in a 96-well plate and grown overnight. (one set per assay) to clear 96-well microplates. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. Cell line development teams will realize savings on time and resources, as well as faster time-to-market by utilizing a single technology for measuring a critical quality attribute duringOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. x. Contact our support team with your questions regarding Molecular Devices products and applications. DynaPro ™ Plate Reader III. Plate Storage Solutions for Liquid Handling Automation Remote Lab Management. We would like to show you a description here but the site won’t allow us. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. Place the microplate in the drawer. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. 305 reference level saturation. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. 2 pM 384–well: 3 pM 96–well: 2 pM 384–well: 4 pM 1 0200-7012POS Glow for 96-, 384-, and 1536-well plates Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well…. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. 5% at 1. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Enter the name of the plate type i. 3% in white 96- and 384-well microplates < 3 x 10-5 (white plates) < 3 x. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. This can increase throughput and efficiency, thus saving time and space in the lab. • To avoid removing loosely attached cells don’t wash if the background signal is not too highTeams measured absorbance at 600 nm (OD600) and GFP in a plate reader from four technical replicates per biological replicate (for a total of eight replicates and fitting on a 96-well plate) at. Category 2 and 3 microplate readers use gradient heating as well. ZERO BIAS - scores, article reviews, protocol conditions and moreThe VersaMax Absorbance Microplate Reader measures the optical density (OD) of samples at selected wavelengths in a number of reading modes. Plate formats 6, 12, 24, 48, 96, 384 wells Light source Xenon flash lamp (1 joule/flash) Detector Photomultiplier (R-3896). Four wash/rinse bottles are included and configurable for both 96- and 384. $14,995. 1. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Published Dec 16, 2020 | Updated Jun 22, 2023. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Temperature variation ± 0. Features: Standard back plate for use with hand sanitizer, hand lotion, or foam dispensers. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED. 1 - 32 of 54 Sort View Show > Well Plate Reader Accessory PerkinElmer For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro. #iD5-STD). Morphology and fluorescence gates were set to select the best 96 clones secreting IgG antibodies. The equation that allows one to calculate absorbance from % transmittance is. 45 µm PVDF. Selects either 96-well or 384-well plate for a stand-alone read. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. Sample by eye within . Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. Molecular Devices delivers a robust screening platform to address real-time kinetic cell-based assays with the FLIPR Tetra® System. 2. 4 decades . This reader can also be used as a standard. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. #E010) Pyroplate 96-well microplate (ACC cat. $542. Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. 000 ABS • Resolution: 0. It is designed to work seamlessly with a variety of 96 well microplate readers, including popular brands such as Agilent™, Berthold™, Biotek™, BMG LABTECH™, Molecular Devices™, Perkin Elmer™, Promega™, Tecan™, and. 96-well microplate compatible Well read area range: D4. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384. The UV-Plate is compatible with the following plate readers: Bio-Tek PowerWave™ 200 Bio-Tek µQuant™ BioRad UltraMark™ Molecular Devices SPECTRAmax® 190, 250, 340PC, and Plus Ordering Information Cat. Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. A standard curve consisting of MCF7 cells seeded from 20 to 20,000 per well in a 96-well plate was linear across the entire range with r 2 = 0. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. SoftMax Pro Software minimizes setup time for detection and automates standard curve fitting and sample quantitation. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. 150 µL of deionized water was pipetted into each well of a clean 96-well plate. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. 1. Used. The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. UV Plate 96 Well, Flat Bottom, Cs 50. It features a modular design that allows users to add or change detection cartridges according to their needs. 25, 3. Two high-energy xenon flash lamps: Reading capabilities. Ultraviolet transparent multiwell plates from GreinerThe Accuris SmartReader UV-Vis (model MR9610) is a welcome addition to any lab that is routinely measuring concentration or absorbance in 96 and/or 384 well plates. 1. Important things to consider when purchasing a multimode microplate reader. The Fluorometric Imaging Plate Reader (FLIPR®; Molecular Devices, Sunnyvale, CA) has made a significant contribution to drug discovery programs in the pharmaceutical industry since the first commercial instruments were introduced 9 yr ago.