The plate reader. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. Figure 2-23: Bottle holder To install the bottle holder: 1. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. FI assay. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. SpectraMax M2. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. 8 foot) 4400-0002 Power cord, 1 meter (3. Choose from a three- (M3), four- (M4), orThere were 12 replicate wells per dilution in both 96-well and 384- well plates. SpectraTest FL1 Fluorescence Validation . Add to Cart. Type. PC software (SkanIt software) Dimensions (D x W x H) 58 x 53 x 51 cm (23 x 21 x 20 in) Weight. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. Prior to fluorescence analysis, the lid from the OmniTray plate was removed. The opaque walls prevent stray light from being detected from neighbouring wells for best accuracy. A whole well fluorescence imaging system that can collect information from all cells inside the well, from 6- to 384-well formats. Flexibility to read 6- to 384-well plates. 00 Free shippingResults show a linear relationship between fluorescence intensity and cell number (Fig. , a positive control). SpectraMax M2 Readers provide for easy conversion and optimization of very-low-throughput to medium-high-throughput assays, faster, more precise results and reagent savings. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Offer. From advanced multimode luminescence, fluorescence, absorbance, BRET and FRET capabilities to dedicated microplate or single-tube luminometers, there is a GloMax® system for every lab and. M2. The plate reader offers a fast, simple readout of average fluorescence per well, while the cytometer gives more detailed information, including total cell number and. Figure 1. 0 × 107) RLU 0. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard plate reader applications, protecting your initial investment. Microplate Readers Biotek ELx808 Microplate Reader Absorbance. By employing dyes that specifically bind to nucleic acids, DNA/RNA concentrations can also be quantified using fluorescence detection. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. 0 out of 5 $ 7,699. Endpoint, kinetic, spectrum. 0 × 103 to 2. spr * SpectraMax i3(x) SpectraTest ABS2 v1. fluorescence intensity (Figure 6). spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. 0 out of 5 $ 2,999. 2. Tecan Infinite F200 Fluorescence Microplate Reader. Microplate Readers Include the M3, M4, M5, and M5. 04 to 3. Next add 100 µl of workingThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. ) Identical data and curves were generated from Gemini EM and SpectraMax M2 but are not shown here. Molecular Devices i3x. type: Clear: Add to Cart. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading. B. Multimode Reader Comparison Chart. spectramax id5, spectramax id3 multi-mode microplate reader, spectramax id3, sof Created Date: 8/20/2020 9:11:23. For paper-based measurements, we used the Milenia HybriDetect 1 (TwistDx) lateral flow system, as per the manufacturer's. The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. 5008531B 3 Contents Chapter1:SpectraTestMulti-ModeValidationPlateOverview 4 Multi-ModeProtocols 6 Chapter2:GettingStarted 8 CertificateofCalibration 8SpectraMax M2. Endpoint, kinetic, spectrum and area-well. . SpectraMax M2 Readers provide for easy conversion and optimization of very-low-throughput to medium-high-throughput assays, faster, more precise results and reagent savings. SpectraMax M5), place the adapter in the plate carriage. Add to Cart. 6 % of reading 4. Spectramax Multi-Mode Microplate Reader Performance Specifications. SoftMax Pro Software has been integrated by many leading robotics and LIMS partners, enabling both data analysis and. ZERO BIAS - scores, article reviews, protocol conditions and moreFluorescence Filter with holder 625nm BW 35nm (Molecular Devices cat. 0 out of 5. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. High tolerance for contaminants. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. Condition: Used - Good. 627. Perkin Elmer EnVision 2104 Multilabel Plate Reader, Perkin Elmer, Envision Plate Reader. Reading Capabilities: cuvette or microplate. #4513: Plate Reader: A multi-detection microplate reader configured to measure the output of the UDP tracer is required. Shop all used microplate readers and parts from a variety of manufacturers. Using standard fluorescence optimization can take hours or days to determine the best wavelengths to use for excitation. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. 40266 Email: drugdiscoverytech@lifetech. Figure 2. Five preferred detection modes. Microplate Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. Absorbance (monochromator) Fluorescence (monochromator; FI, TR, FP) Luminescence; Molecular Devices Spectramax M2. Molecular devices SpectraMax M5 Pdf User Manuals. Microplate Readers Biotek Synergy 2 Plate Reader Multi-Mode. 0) OD (0. . 40266 Email: drugdiscoverytech@lifetech. Data were analyzed with SoftMax ® Pro Software and graphed using a 4-parameter curve fit. Select the lab user profile. Filters: Excitation and emission slides for 4 filters each. read matrix, spectral scanning and detection of plate formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. Therefore, ADP production is proportional to an increase in fluorescence. The chamber. luminescence, and fluorescence microplate readers, and the SpectraMax® Paradigm® Multi-Mode Detection Platform. In addition, the SpectraMax iD5SpectraMax Microplate Readers. WinKQCL ® Software guides the user through the process of setting up a microplate template and running an assay to determine the amount of endotoxin in a sample solution. spr SpectraMax iD5 SpectraTest ABS2 v1. Category 2 and 3 microplate readers use gradient heating as well. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. We here describe a fluorescence resonance energy transfer (FRET) based fluorescence plate reader assay to monitor the interaction between fluorescently tagged sortilin and p75 NTR in live cells. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. Plate reader set to 50 reads. Detection modalities include absorbance (UV-Vis Abs) and. SpectraMax ABS Plus SpectraTest ABS2 v1. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 0 × 103 to 2. Microplate Readers Molecular Devices SpectraMax M2 Multimode Plate Reader. Additionally, by issuing a single read command, combined absorbance and fluorescence intensity assays can be run. PerkinElmer’s new EnSight Multimode Plate Reader reads label-free Corning® Epic® technology (in addition to standard labeling technologies) and is appropriate when multiple assay types are more important than speed. was 1. Insert the snorkel into the bottle and then insert the snorkel into the snorkel clamp. Compact and tunable absorbance microplate readers DATA SHEET. Based on the SpectraMax M5e platform, this five-mode reader addresses multiple application technologies including: absorbance, fluorescenceSeller assumes all responsibility for this listing. SpectraMax Quant dsDNA Assay Kits. 0 out of 5 $ 3,895. Fluorescence is measured at time zero and again after one-hour of incubation at 37°C in a fluorescence microplate reader. 0 out of 5 $ 2,999-20%. Third this recommendation. on SpectraMax M2 fluorescence plate reader (Molecular Devices, CA, USA): excitation wavelength 320nm, emission wavelength 470nm. 0077 OD 0. Multimode Readers Comparison Chart. Microscopes and Microplate Instrumentation. standard spectral absorbance, fluorescence, and luminescence detection. FI assay. Analyze the data Step 1: After the microplate has been read, theFluorescence analysis was performed using a SpectraMax iD3 multi-mode plate reader (Molecular Devices LLC, U. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader and Absorbance plate. The OliGreen assay from Molecular Probes, when run on a Gemini XS or Gemini EM fluorescence microplate reader with SoftMax Pro software, is a quick, sensitive detection method for single-stranded DNA. Highly selective for dsDNA over ssDNA or RNA. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 Instrument settings used with the SpectraMax M5 Microplate Reader. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. SoftMax Pro. Plate stacker and robot integration The SpectraMax M2 and M2e readers can be integrated with the A microplate reader that can detect two or more applications is considered a multi-mode plate reader. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Instruments are configurable for the assay-specific detection modes and performance your laboratory needs. 00. 2 only) SpectraMax M-series Microplate readers. The assay is carried out in a standard 96-well plate. With optimized reagents, option to operate the readers. It does not work as expected. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. SpectraMax readers are equipped with PathCheck Technology, which measures the pathlength in each well of a microplate and normalizes the absorbance. MOLECULAR DEVICES SPECTRA MAX GEMINI XS MICRO. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). In the presence of ACE, the PL intensity was increasing by time, while the PL intensity sustained a low and stable fluorescence in 3 hours in the absence of ACE. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13Fluorescence Microplate Readers. 0 software was used to handle data analysis and control each instrument. The SoftMax Pro Software validation package and IQ/OQ validation protocols include tools for FDA 21 CFR Part 11 compliance. 4. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. Learn how you can utilize microplate readers to drive landmark discoveries. Documentation:Plate readers that are used to detect luminescence, fluorescence and absorbance signals can vary in sensitivity (Figure 2). The purple holder is used for reading non-transparent plates from the top (e. Microplate Reader Shelf. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. Page 28 Chapter 5: Read Modes and Read Types 1. E Models. 0 × 103 to 2. Adjusting fluorescence gain on microplate readers. The percent The percent (4) Asiaticoside reverses M2 phenotype macrophage polarization-evoked osteosarcoma cell malignant behaviour by TRAF6/NF-κB inhibition Pharmaceutical Biology December 31, 2022 Dang-ke. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Read More. This plate reader is capable of monitoring UV and visible absorbencies, fluorescence, and luminescence. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command. The fluorescence intensity was captured with a SpectraMax Paradigm Multi-Mode Microplate Reader (Molecular Devices, LLC, San Jose, CA, USA) at 588 nm excitation and 633 (18) SARS-CoV-2 antibody signatures robustly predict diverse antiviral functions relevant for convalescent plasma therapy medRxiv September 16, 2022 Harini Natarajan. Add to Cart. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. D 3 1. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. The Molecular Devices SpectraMax i3x Microplate Reader is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreThe SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. The FI assay was performed using the SpectraMax ® M2 Multi-Mode Microplate Reader. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Data fromSpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E. SpectraMax Microplate Readers. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers are as Fluorescence Validation Test Plates provide validation of the system’s optical characteristics. Conclusions. 53 - 60 kg, (117-132 lb), depending on configuration. 1 cm) Weight 27 lb. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2. 3 foot) See. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. 304 signal level saturation. Fig 2: Enzymatic response of synthetic compounds. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. Plate chamber design of the SpectraMax category 1 microplate readers. The instrument is located in room 2131 at 300 George Street. Type Clear: Add to Cart. Luminescence was read on the SpectraMax i3x reader and the SpectraMax L reader. made for this assay plate. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. Important things to consider when purchasing a multimode microplate reader. 25 to 3. The SpectraTest absorbance test plate (Abs-1) can be used by VersaMax, SpectraMax, & M2 readers. begin reading microplates with seven mouse clicks. Absorbance cuvette port. 25 to 3. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Appendix. It may be named "Pothos". SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. SpectraMax Quant dsDNA Assay Kits. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. SpectraMax Microplate Readers. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing. Multimode Readers Comparison Chart. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Maximum signal with lowest background. After washing with PBS, the fluorescence intensities for each staining were analyzed with a fluorescence plate reader (SpectraMax M2). microplate readers provide flexibility, performance, and ease of use for a variety of microplate assays. (in addition to fluorescence imaging) on the SpectraMax i3x Multi-Mode Microplate Reader. SpectraMax M2E Multimode plate reader with cuvette option, ABS and FLR reading and extended range. Compatible automation solutions for SpectraMax Readers. Plate background OD values of disposable UV microplates vary slightly between lots. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreThe SpectraMax iD3 reader measures absorbance, fluorescence, and luminescence. 5,112,134 5,766,875. To avoid these issues and provide the best possible dynamic range between the highest and the lowest measurement values of your assay, fluorescence gain is typically adjusted on the sample with the expected highest signal output (e. When a feature or capability applies to only certain readers, this exception is noted. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and. Plate Formats: 6, 12, 24, 48, 96, 384 wells: Light Source: Xenon Flash Lamp (1 joule/flash)The SpectraMax® iD3 Multi Mode Microplate . 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. assays, we can offer a microplate reader solution to help meet the. The kit includes everything you need: reagents, buffers and a microplate. D 47 7. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western. The SpectraMax M2 reader can be easily converted and optimized for very low-throughput to medium-high throughput analysis, faster, more accurate results and saving reagents. Microplate Readers. Real-time experimental control (REC) that automatically activates a procedure as soon as a specific data value is reached. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. , contact. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Fig 2: Enzymatic response of synthetic compounds. 49 µM,. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. 00. LuminescencePhotometricPerformance. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Molecular Devices SpectraMax M2. S. 25 to 3. 1. Reviews. 315 can't find zero order. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. For example, microplates containing different samples can be read using the Tecan Infinite® M1000 Pro Plate Reader. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). Shop now. Reader Documentation Version No. Many of the following instruments are multimode readers that, in addition to luminescence detection, can also be used to measure UV. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3. Note: In this user guide, SpectraMax ® refers to several SpectraMax . 25 fmol/well 384-well plate) Filters/Mirrors: Fluorescein 1 pM typical (0. Fluorescence measurements were acquired at 10 min intervals (ex: 485 nm and em: 535 nm) in a SpectraMax M2 fluorescence plate reader (Molecular Devices) operated in the 384-well microplate format. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. 0 × 107) RLU 0. 67. NanoQuant plate. The LysoTracker fluorescence at ex/em 574/594 was measured on a Spectramax i3x plate reader (Molecular Devices). What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. 1 New Reader Support 1. SpectraMax® M2 SpectraMax® M2e Multi-Mode Microplate Readers User Guide 0112-0102 E October 2018 SpectraMax M2 and M2e Microplate Reader User. The SpectraMax Plus 384 Microplate Reader for. D 59 A. The SpectraMax microplate readers support a wide variety of assay categories including . free tracer decreases, resulting in an overall increase in RFU values. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck® Sensor. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Microplate readers’ standard detection modes are. Description 1. Item Description Sensitivity 10amol/wellalkalinephosphate200µL Wavelengthrange 250-850nm Crosstalk <0. The Biophysical Resource at Keck Facility offers the fluorescence plate reader as an "open access" instrument that can be used by trained users. This enables the standard curve to be plotted and sample concentrations to be calculated automatically by SoftMax. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. D 3 1. In the M2, M5 and Plus 384, the light. Cells in each image are identified by SoftMax Pro Software and cell-by-cellfluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). View online or download Molecular devices SpectraMax M5 User Manual. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF. The SPECTRAmax® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics that permit experimental determinations at low-level detection limits. Enables accurate fluorescence data with every run. The superior optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) that reduces background noise for excellent sensitivity and a wide dynamic range. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. . Microplate Readers. In the second assay, HepG2 cells were plated into a 96-well clear-bottom, blackwalled plate at 10,000 cells/well and incubated overnight at 37°C. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. the reader’s stated range. The SpectraMax iD5 reader rapidly performs absorbance measurements that are used to plot standard curves and calculate sample concentrations using a preconfigured protocol in SoftMax Pro Software. When a. It can measure absorbance from 230 -1000 nm. When a. Factors that contribute to an instrument’s sensitivity, or limit of detection, include background noise from the detector and electronics, the type of detector that is used, and the instrument’s configuration and. A decrease in fluorescence with increasing staurosporine concentration was detected with the SpectraMax iD3 reader. We therefore gathered salient. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize. Type Clear: Add to Cart. SpectraMax M2 and use thereof is covered by issued (U. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideELISA Plate Readers / Microplate Readers. A microplate reader as individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. 0 out of 5 $ 14,499 $ 13,995-13%. Microplate Reader Shelf. For a complete list of the instruments supported by this release of theThe HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). The microplate reader will read the plate and the relative fluorescence units will be displayed in the Plate section. A solution for low volume applications. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Set up a plate section for a second emission scan as in Step C above, except for the added Emission Cutoff Filter. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. The chamber. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). com Setup for Omnia® assays on SpectraMax®. This opens the Settings window. SPECTRAmax GEMINI-XS spectrofluorometer was able to detect a specific GFP signal at all cell numbers as determined by One Way Analysis of Variance. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Microplate Readers Molecular Devices SpectraMax M2 Multimode Plate Reader. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. ₹ 1,80,000 Get Latest Price. (a) Chemical structures of 1 (HADP) and its analogues 2–4. D 59 A. Plate Shaking Linear, orbital, double-orbital Dimensions (H x W x D) 12 x 15 x 15 in. Multimode Microplate Readers. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Align the two slots on the bottle holder with the two pegs on the rail. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. On the SpectraMax iD5 reader, install the 320/100 excitation filter and 616/10 and 665/10 emission filters, and then select these filters from the Excitation and Emission dropdown wavelength menus. Usage/Application: Lab. flexibility of monochromator-based systems. Note: Check the Applies to section at the bottom of the page to make this is the proper driver for your plate reader. Step 2: Click SoftMax Pro’s Read button. The plate is set up for reading and images are acquired according to specified parameters. Skip to main content. Using a black. 45 s, and a 1,536-well plate in 135 s (minimum times) Interface. The SpectraMax Paradigm Detection Cartridge design utilizes an energy intensity optimization technique that allows the user to read up to seven logs of sample concentration in a single pass of a plate without any manual intervention. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. 2. The M4 Reader offers the same modes as the M3 Reader, plus time-resolved fluorescence, and can be upgraded to include fluorescence polarization. S. . Brand: MOLECULAR DEVICES. D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive. Microplate Reader, Multimode. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. 119. Biotek Synergy HT Microplate Reader Multi-Mode. Optimized Settings to Validate the Molecular Devices SpectraMax® M2/M2e Microplate Readers with the Transcreener® Fluorescence Intensity Assays. 2018. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. The IC50s by cell rounding and LysoTracker assays were 0. Selecting your. TRF as Secondary Detection Mode: TRF is supported as a secondary detection mode by these readers: Gemini XS, EM, XPS, M2, & FlexStation. Driver installation. B. Peeking into a research lab today, it would be rare to see a line-up of microplate readers on a bench, each capable of performing only a single type of measurement. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize. Figure 6: Transcreener ADP2 FI assay principle. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by issuing a single 1) Select the correct plate and the wells that you want to measure. Page 28 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 4. The plate is set up for reading and images are acquired according to specified parameters. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Multimode microplate readers deliver both performance and economy. Microplate Formats: 6, 12, 24, 48, 96, 384. An assay plate containing a 15-point standard curve was read on the SpectraMax. D 3 1.