With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3 allows users to customize workflow to perfectly. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. Here, we demonstrate performance of the SpectraMax® iD3 Multi-Mode Microplate Reader for assays measuring intrinsic tryptophan fluorescence. The chamber contains springs that position the cuvette in the proper alignment for a read. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. This variable pathlength makes it dif Þcult to perform extinction-based assays and confusing to compare results between microplate readers and spectrophotometers. 3) At the bottom of the strip, click on "Show. SAN JOSE, CA — Molecular Devices, LLC. 314 low lamp level warning. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostPhysical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein. ” Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. All . These lab workhorses are used in drug discovery, research, bioassay. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Offer Ends In: 2 Days 10: 40: 19. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. The cuvette must be in place when you read the plate. The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. Minimize manual manipulation of samples and data processing, and improve laboratory efficiency. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingevery Gemini EM Reader. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Five preferred detection modes. We would like to show you a description here but the site won’t allow us. High sensitivity is demonstrated with a tryptophan standard curve, and a shift in peak emission is shown using a lysozyme denaturation assay. The instruments affected are the SpectraMax ® L Microplate Reader and the StakMax® Microplate Handling System. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. It can run samples based on pre-defined. The Molecular Devices Gemini XS Fluorescent Microplate Reader lets you experiment with a variety of fluorophores and can even find the best excitation and emission wavelengths for new assays. Self Service Available. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. Users can perform spectral scans from 250 nm to 750. We would like to show you a description here but the site won’t allow us. Step 1: Open the SoftMax Pro IMAP FP protocol. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Three low volume 24-well bottom slides. SpectraMax ® M2 Multi-Mode Microplate Reader. Published Dec 16, 2020 | Updated May 19, 2023. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. This is equivalent to having 511 built-in filters, The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. Chapter5:ReadModesandReadTypes 0112-0115J 27 Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. Figure 1. 3) This warranty. Or scan up to 384 wells at any time to determine the best assay conditions. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 μL sample volume in a single read. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Lift up the lid over the chamber to insert or remove a cuvette. This section contains the following topics:滤光片型光吸收微孔板读板机（酶标仪），简单易用，性价比高，检测结果精确. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. SpectraMax Mini Plug-In for SoftMax Pro 7. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. 20 μL of Component A was added to each well of the microplate. 100 fmol 384-well (75 µL) 0. Selection of Water Constant in PathCheck settings. Reader modes are customized to your specific applications with options to upgrade easily. Instrument settings used with the SpectraMax M5 Microplate Reader. Fully tested with validations, warranty. Molecular Devices SpectraDrop ™ Micro-Volume Microplate allows users to read up to 64 samples per plate on SpectraMax ® Microplate Readers, with sample volumes as low as 2 µL. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Sale. The drawing shows a standard microplate of 15 mm height covered with a lid. For readers without a cuvette port, simply check the box next to ‘PathCheck’. Compact and tunable absorbance microplate readers DATA SHEET. microplate reader. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path length. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. For the calculation of the ratiometric biosensor signal, the emission intensity at 510 nm was recorded upon an excitation at 380 nm and 470 nm and the former was divided by the latter (Exc. 304 signal level saturation. . SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. Figure 1. An enhancer is provided in the kit where applicable to further reduce background fluorescence and to increase the. The Gemini EM can sample a variety of formats, from 6- to 384-well. 7°C and the bottom to 36. 303 unable to cal dark current. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Plate chamber design of the SpectraMax category 1 microplate readers. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. 0 out of 5 $ 23,995 $ 22,495. Large, high resolution touchscreen interface allows you to easily set up methods, run experiments or view tutorial videos without the need for a dedicated computer workstation. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. Fluorescence Microplate Reader Comparison Chart. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. In addition, the SpectraMax® iD5 can be expanded to include bottom. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and. com. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. 1. 997 (n = 3). . Avoid losing high signal. 72 MB. their consistent performance, reliability and durability. Lower limits of detection for ATP determined using the ATPliteAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. IMAP FP Settings in SoftMax Pro SpectraMax M5 Multi-Mode Microplate Reader. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. Align the two slots on the bottle holder with the two pegs on the rail. Multimode Microplate Readers. Additionally, user-installable options allow the SpectraMax i3 System to. Add to Cart. 0 out of 5The SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Wide range of applications. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Example: [Drawer]. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. Download the SpectraMax Mini Reader Plug-in and associated guides. 0200-6263. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. Each sample has a discrete sample beam and reference beam so that each well is measured directly. Use the provided 3. 100 readings per well gives optimal Z’ values for this assay. For biological applications involving small samples, such as purificationThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The SpectraMax 190 reader can be integrated with Molecular . 470 nm). 315 can't find zero order. Improve your lab’s efficiency, get more data, and decrease time to discovery with an automated microplate washer and reader solution: The SpectraMax® Mini Multi-Mode. Microplate Readers Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. The instrument uses a paired monochromator and xenon flash. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. When the user applies a set temperature of 37°C, the top is heated to 37. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. Examples: “Press carefully to engage,” “ Do not. The Spectramax 250s xenon flash lamp and grating monochromator facilitates direct UV measurement of DNA, RNA, and protein in microplates. 0 out of 5 $ 9,995 $ 9,495. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. The Gemini XS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. Molecular Devices. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Transcreener ® ADP. Microplate reader settings. The analysis capabilities of the software provide quantitation in an easy-to-read, user customizable report format. Microplate automation and detection instruments for walk away operation and high quality results. Table 1. This system includes the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Kit and image acquisition software powered by SoftMax Pro® Software. Blank subtraction and standard curve fitting were done automatically by the software. Fluorescence analysis of the colonies from the dilution series was performed in a microplate reader (SpectraMax iD3). The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. Tecan Infinite Microplate Reader. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. When read on microplate readers, the SpectraMax Quant dsDNA Assay Kits enable optimal results and increased assay throughput. For more sample throughput, both readersThe SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. SpectraMax 250 User. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 5 12. The drawing shows a standard microplate of 15 mm height covered with a lid. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. In addition, the SpectraMax® iD5 can be expanded to include bottom. Using the. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Microplate Readers BioTek Epoch 2 Microplate Reader. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate312 gain calibration failed. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. 7°C (gradient heating design). Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. begin reading microplates with seven mouse clicks. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published0200-6262. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. 0 out of 5 $ 5,999. Read More. Figure 6: Transcreener ADP2 FI assay principle. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. microplate reader. SpectraMax® Microplate Readers and SoftMax® Pro Software. 1. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Introduction. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications. . Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as ELISA, protein detection, and drug screening assays. SpectraMax Plus: SpectraMax Plus. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMinimum volume and maximum throughput with 96- or 384-well microplate compatibility. SpectraMax L Microplate ReaderThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The University of Rouen in Normandy uses the SpectraMax iD3 and. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. Add to Cart. limit of detection (LLD) for ATP was estimated for each microplate reader using the . The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. The SpectraMax 190 reader can be integrated with Molecular . The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. 5 12. DNA and RNA measurements in SpectraMax® microplateSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Note that this procedure is only for the SpectraMax L model. Results are shown in Table 1. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow toFlexStation® 3 Multi-Mode Microplate Reader. We would like to show you a description here but the site won’t allow us. The SpectraMax L Luminescence Microplate Reader with the variable-volume injectors option (10 µL to 100 µL) can measure both flash and glow luminescence. Italic and boldface type are used for emphasis. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Examples: “Press carefully to engage,” “ Do not press down. Our SpectraTest® ABS2, FL1, LM1, SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86 The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Read one sample or up to 384 inThe SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. In addition, the iD5 reader measures TRF and FP. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. Download PDF. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. 313 reference gain check fail. It can run samples based on pre-defined protocols and standard filter modes, and is compatible with ELISA and protein quantification assays. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. The advanced optical and. edu) to be added as a trained user in iLab. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. 1 software Download page. When the user applies a set temperature of 37°C, the top is heated to 37. All stand-alone instrument functions can be controlled using the software. 76358-624. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. 6 for further experiment, so I'm confused which i would choose for OD value determination. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Absorbance (optical density, OD) was read at 570 nm and 605. Cuvette Microplate wells. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. The SpectraMax Quant dsDNA Assay Kits consist of three products, each designed for a specific range of DNA concentrations and optimized for use with fluorescence microplate readers such as the SpectraMax microplate reader (Table 1). 012 fmol 0. Please refer to the Obsolete Microplate Reader Models for more information. Both readers feature an 8-channel read head for fast reads and a subset feature our patented PathCheck™ Sensor. Category 2 and 3 microplate readers use gradient heating as well. Compact designs. The SpectraMax® i3 is Molecular Devices latest multi-mode microplate reader which delivers several applications including; Ultra-Violet to visible absorbance detection, fluorescence intensity and multi-colour luminescence. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. Page 28 Chapter 5: Read Modes and Read Types 1. 303 unable to cal dark current. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. western blot membrane detection in a multimode microplate reader platform. Use similar settings for other Molecular Devices readers with fluorescence detection mode. It measures the optical density (OD) of samples in both 96- and 384-well. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The SpectraMax® ABS Plus Microplate Reader can run . Detailed specifications of each model reader are reported in Section 7, “Specifications”. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. Also see:. Examples: “Press carefully to engage,” “ Do not. Read one sample or up to 384 in0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. 1. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. We use Spectramax M5 microplate reader in our lab. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. The screw remains inside the instrument. Category 2 and 3 microplate readers use gradient heating as well. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. 专为科研检测平台量身打造的灵活且强大的小型微孔板读板机（酶标仪），具备终点法和动力学检测模式，标准配置6块滤光片，覆盖蛋白定量、细胞活力、农药残留、ELISA实验等. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. SpectraMax iD5 Multi-Mode Microplate Reader Optional, for SpectraMax iD5 reader: Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The following document is intended to demonstrate setup of this instrument. The calculated LOQ values were 0. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Omnia® Compatible Microplate Reader Documentation Version No. Order Now. The SpectraMax iD3 reader is fully compatible with the SpectraDropTM Micro Volume Microplate, enabling quantitation of precious low volume samples. More Product Information. Download PDF versions of the SpectraMax Microplate Reader User Guides. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. Detailed specifications of each model reader are reported in Section 7, “Specifications”. Brochure. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 314 low lamp level warning. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. Unlock your i3/i3x instrument after shipment. In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. Wide range of applications The VersaMax reader covers. Assays vary depending on temperature or pH and luminescence microplate reader validation DATA SHEET. Results are shown in Table 1. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. Compare plate readers The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Nuclear Receptor. Cell Analysis. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. The SpectraMax ABS Plus Microplate Reader is a compact and adaptive microplate reader capable of quantitating nucleic acid and protein samples in various formats such as cuvettes or microplates. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. 1. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual. 2. The optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual monochromators. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. . Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started immediately, using the preconfigured Kinetic Turbidimetric LAL protocol in SoftMax® Pro Software, with. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. A preconfigured protocol is available in the software to. . begin reading microplates with seven mouse clicks. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. 4 μL DNA standards. SpectraDrop High-Throughput Screening (HTS) Kit. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Step 1: Turn on your microplate reader. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3 and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most publishedi3x. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. 313 reference gain check fail. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Download PDF. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. Microplate readers’ standard detection modes. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. 306 plate air cal. San Jose, Calif. Assays can be better optimized by scanning across. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. With optimized reagents, option to operate the. 2. The plate reader can measure multiple readouts, including UV. For biological applications involving small samples,Minimum volume and maximum throughput with 96- or 384-well microplate compatibility. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for. Find regional support. Other technologies such as imaging. This is equivalent to having 511 built-in filters,The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. , a. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. We would like to show you a description here but the site won’t allow us. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Dual-Glo luciferase assay system (Promega Cat. 305 reference level saturation. 2 only) SpectraMax M-series Microplate readers. Bookings are free, but you will need to use iLab to book. 1. Create custom fluorescence assays without having to purchase expensive filter sets. Intended Use/Discipline:Biological. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has today launched its. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. It also integrates with the.