Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. Spectranet customer portalSpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. The device can be upgraded to include capabilities for Western blotting, imaging and injection. SpectraMax i3x Multi-Mode Detection Platform User Guide. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. The treatments were. See SpectraMax® Microplate Reader User Manual Download Page. Expand your lab’s capabilities to include flash applications such as dual luciferase and. Turbidity measurements were performed with a Spectra Max® i3x microplate reader to study the effect of oxidation on the kinetics of sFN formation. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. SpectraMax i3x and Paradigm readers require the FP-FLUO. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. 315 can't find zero order. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Besides, it’s possible to examine each page of the guide singly by using the scroll bar. Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. Fourier transform infrared (FT-IR) spectra of cCQD were obtained on a. Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Settings not required by a reader are indicated by ‘---’. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. QBT Fatty Acid Transporter Assay. Register to download your free eBook today. MTT was purchased from Sigma-Aldrich (St. 315 can't find zero order. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. User-upgradeable detection modules allow you to expand the capabilities. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). 2. The drawing shows a standard microplate of 15 mm height covered with a lid. The fluorescence spectra were acquired using a microplate reader (Spectra Max i3x; Molecular Devices, USA). Prepare your i3/i3x instrument for shipment. Contact Local Team. 304 signal level saturation. Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Amplitude : 1. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). To read HTRF®, the SpectraMax i3x® must be first equipped with the SpectraMax i3x® Cisbio HTRF® cartridge, which enables the simultaneous measurement of both 620nm donor and 520 nm acceptor emissions. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. 1. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Table 1. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. pathways and protein activation and expression in one system. Price from $9. ZERO BIAS - scores, article reviews, protocol conditions and more. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. Spectranet customer portalThe Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). . CRITICAL STEP Avoid air bubbles; reverse pipetting the luciferase reagent into the plate is a good way to achieve this. Spectramax I3x, supplied by Danaher Inc, used in various techniques. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks. Spectranet customer portalWatch this video to get an introduction to the Injector-Ready SpectraMax® i3x Multi-Mode Microplate Reader System. Eliminate time-dependent substrate addition steps. 303 unable to cal dark current. Item SpectraMax i3x Multi-Mode Microplate Readers; Company Molecular Devices LLC; Catalog Number SpectraMax i3x <Disabled> This product is no longer available on Biocompare. Fluorescence Reader. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. 0. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. *On the SpectraMax i3x reader only. Recommendations for SpectraMax i3/i3x plate readers The Molecular Devices SpectraMax i3/i3x allows use of the pH-Xtra Glycolysis Assay with time-resolved fluorescence (TRF) detection. 00800 665 32860. 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. How to properly unlock iD series instrument. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. 660-665 Eskdale Road. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. The Molecular Devices SpectraMax i3x Microplate Reader is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. The UV-Vis spectroscopy was measured on a TU-1900Beijing PuXi instrument. Analysis. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Patented user-exchangeable cartridge design expands the system's detection capability making it. Average 98 stars, based on 1 article reviews. StainFree Cell Detection Algorithm. each new lot of plates, to ensure that the center of each well is read. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,i3x Multi-Mode Microplate Reader. The plate was shaken and incubated in the dark for 10 minutes. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. 吸収度マイクロプレートリーダー SpectraMax® Mini. Ease of use. View online or download 1 Manuals for Molecular Devices SpectraMax i3x. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Example: [Drawer]. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. Learn how the SpectraMax Paradigm Multi-Mode Detection Platform with Tunable Wavelength (TUNE) Detection Cartridge combines the flexibility of wavelength scanning with the high sensitivity of filters. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. 3) At the bottom of the strip, click on "Show. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. 58% for the detection module and 2. Find regional support. See5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideMolecular Interactions. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. 200-800 nm using Spectra Max i3X microplate reader. 2. Capture Flash Assays with Ease. Actual shake speed is based on the microplate format. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. SpectraMax i3x. Italic and boldface type are used for emphasis. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The protocols are also available on the SoftMax Pro Software protocol sharing. Multimode Reader Comparison Chart. Microplate optimization should be performed the first time the assay is run, and subsequently for . Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Key features. Besides, it’s possible to examine each page of the guide singly by using the scroll bar. Automated for ease of use. 0 mm hex key. Synthesis of NORP Synthesis of compound 1. In addition, Image J was used to quantify the. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. We would like to show you a description here but the site won’t allow us. Promega. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. ZERO BIAS - scores, article reviews, protocol conditions and more. 5577 Web: Email: [email protected] SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. As. Explore a wealth of applications in one future-ready system that measures spectral-based absorbance, fluorescence, and luminescence. Prepare your iD series instrument for shipment. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. Bookings are free, but you will need to use iLab to book. Description 1. Downloads. More Product Information. Figure 1. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Remove the transport bracket and store the retaining screws in the bag that held the 3. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. The SpectraMax i2X is used for cell migration, cell. Read height should be optimized for . The fluorescence spectra was obtained on a Spectra Max i3x Multi-Mode Detection Platform. Controls containing all reaction components with either DI . A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Molecular Devicesの全商品を見る. 6 in. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. As. Nuclear Receptor. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. Modularity does provide some measure of forward vision into upcoming or future experimental needs. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Description 1. Louis, MO) and used as received without further purification. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. D 1 1. A: percent GFP-positive cells calculated using the classification feature in the software. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strength and superior sensitivity across the full. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Register to download your free eBook today. To learn more about the device, please watch this short video. Manufacturers try to design their instruments so they can be. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. Prepare your i3/i3x instrument for shipment. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Spectranet customer portal The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Multimode Reader Comparison Chart. comThe SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThe absorbance was recorded on a spectra Max i3x microplate reader (Molecular Devices, USA) at 450 nm. The plate was covered and incubated for one hour at room temperature. Also refer to this Multimode Reader Comparison Chart. Molecular Devices Software. Increased fluorescence performance. Order Now. Spectranet customer portalPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. The dual auto injector module. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The soil suspension (600 µL) and the substrate (L-3,4-dihydroxyphenylalanine, DOPA, 150 uL, 25 mmol L -1 ), were mixed and then added to. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. , 2021). Align the two slots on the bottle holder with the two pegs on the rail. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthThe SpectraMax i3x reader grows with you as your research areas expand. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. 7°C and the bottom to 36. 11. china@moldev. Promega. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. 2. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects and changing objectives, the i Series has you covered. Spectranet customer portalThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. See SpectraMax® Microplate Reader User Manual Download Page. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. User-upgradeable detection modules allow you to expand the capabilities of your microp. 1 mg/mL density of resazurin, protected of the light, incubated for 1 h at room temperature. About the reader setup recommendations The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 4. Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). Shake intensity. Figure 1. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. 3: The cuvette chamber. , 2011). With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Multimode Reader Comparison Chart. 1、卡盒式模块和细胞成像模块的构架检测功能（如FP、TRF、HTRF、AlphaScreen、ScanLater Western Blot等）卡盒模块化设计，仅通过插入卡盒就能可. 2 x 10-14. However, no piece of equipment is immune to breaking down. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves. Published Dec 16, 2020 | Updated Oct 10, 2023. See more, and do more, with the SpectraMax® i3/i3x Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick imaging and analysis of cells and gives you a front-row view of phenotypic changes that accompany cytotoxicity,. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. ATP (“flash” luminescence using . We would like to show you a description here but the site won’t allow us. 312 gain calibration failed. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® Microplate Readers and SoftMax® Pro Software. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. Replicate values were averaged and imported into Design-Expert 8. Anne T. china@moldev. Danaher Inc spectramax i3x. Download PDF. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. SpectraMax i3x 一套系统即可以帮助你通过科学手段解开细胞通路的奥秘，也可以检测并分析出 样品中蛋白质的活性和表达情况。SpectraMax i3x 检测系统可以根据未来实验需求，无需售后工 程师协助即可加入全新检测功能模块，真正做到一次投入终身受益。1) Select the correct plate and the wells that you want to measure. 2 only) SpectraMax M-series Microplate readers. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. Native or oxidized AN (40 μM) and FN (0. techsupport. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 7. Cell counters for accurate and. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. 4. Fluorescence emission spectra were collected on an ELISA molecular system (Spectra Max i3x). Quickly and easily expand the capabilities of your SpectraMax Paradigm or i3x Multi-Mode Microplate reader to include Tunable Wavelength detection using the. Prepare your i3/i3x instrument for shipment. 12 mg/mL) of polysaccharides were prepared after addition of glucosum anhydricum for calibration. SpectraMax i3x Multi-Mode Microplate Reader System. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Prime function 20 amol. 1step assay with SpectraMax microplate readers. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. Description 1. 1) Select the correct plate and the wells that you want to measure. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. *On the SpectraMax i3x reader only. com The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. flThe SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. The treatments were. TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthTNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. Relative mitochondrial polarization was expressed as the ratio of emission at 590 nm to that at 530 nm (Shin et al. 3: The cuvette chamber. Bioz Stars score: 98/100, based on 1 PubMed citations. 1. In this application note, we describe how the SpectraMax ® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. Be Future Ready with the SpectraMax i3x Multi-Mode Detection Platform. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. 306 plate air cal fail,low light. 9 x 10-15. It allows you to explore cellular pathways and protein activation and expression in one system. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataThe SpectraMax i3x reader and MiniMax cytometer can be used with FluoroBlok cell culture inserts to directly count cells from high-quality images to measure cellular migration over time. An MPO assay has been performed to quantify the inflammation reduced by the GAR-PLGA-ES100 NPs in the inflamed cells . Supplier Page. SpectraDrop Abs Protein Quant (i3-i3x)Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Prepare your iD series instrument for shipment. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 0. SpectraMax i3x microplate reader and SoftMax Pro Software protocol. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. Figure 4. It allows the user to determine the total concentration of protein in a solution. Table 1. Methods. Get a front row view of your cells. ) wide and 65 cm (25. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. 2 in. Supplier: Molecular Devices. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Note: Additional readers with similar performance are listed. Patented user-exchangeable cartridge design expands the system's detection capability making it. 6 in. Accordingly, we calculated the Laurdan generalized polarization (GP) using the formula GP = (I 435 − I 490)/(I 435 +. 0´ 105 dsD NA (p g/ wel ) R F USpectraMax Glo Steady-Luc 工作液都加 入到微孔板后，震荡摇匀并避光放置 10 min。使用 SpectraMax Glo Steady-Luc Reporter 内置的 SpectraMax Glo Steady- Luc Reporter 实验模板设定程序并通过 SpectraMax i3x 读板机检测发光。检测结 束后，模板会根据数据自动生成一条曲 线。SpectraMax MiniMax 300 细胞成像系统 为 SpectraMax i3x 多功能微孔板检测平台而打造！ • • • • 主要特点 系统全面兼容细胞成像 及微孔板检测技术 独特无标记分析技术无需 对细胞染色即可进行细胞 计数和贴壁度的计算 具有多种检测功能可对 细胞活力及毒性进行全. High resolution X-ray photoelectron spectroscopy (XPS) of cCQD was obtained on a K-Alpha+ (Thermo Fisher Scientific, Massachusetts, USA) spectrometer. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. edu) to be added as a trained user in iLab. Published Dec 16, 2020 | Updated Dec 16, 2020. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. 8 Antibodies and. It allows you to explore cellular pathways and protein activation and expression in one system. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. 0 | 9 reviews | Write your own review Measures Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgradesMolecular Devices SpectraMax i3x Multi-Mode Microplate Platform. 0 mm hex key to remove the six blue retaining screws that secure the transport bracket (B) to the instrument. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. 4 mg mL −1; 1. Sunnyvale, CA, USA) with excitation at 475 nm. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . ATP (“flash” luminescence using . 5 µL anti-cAMP-Cryptate. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreSimilar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). SpectraMax i3Instrumenttovisuallyinspectyoursampleandtoruncell-basedassaysat cellularorwhole-cellresolution. We would like to show you a description here but the site won’t allow us. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. Every SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. SpectraMax® i3x 多功能读板机配置 SpectraMax® MiniMax™ 300 细胞计数 仪，具有 transmitted light (TL)，绿色 (Ex / Em: 460 / 541) 和红色 (Ex / Em: 625 / 713) 荧光通道。MiniMax 细胞计数仪的 SoftMax® Pro 软件可以用来获得各种细 胞的图像并进行分析。本说. Bioz Stars score: 98/100, based on 1 PubMed citations. Pick the best speed, sensitivity, and price for your needs. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. 在SpectraMax i3x和SpectraMax iD5微孔板读板机上使用 SoftMax Pro软件绘制标准曲线，并进行4参数曲线拟合(图 4)，结果见表2。使用EC50评估该测定方法的适用性，EC50值 应低于150 nM。对于两个读板机，EC 50值均低于85 nM，处 于可接受范围内。Cisbio将可接受的信噪比定义为≥. Structured Review. SoftMax Pro GxP Compliance Software. GFP expression imaged on the cytometer. Find regional support. 4 μM), prepared as described in section 2. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . 10, and 0. Red and green fluorescence was then measured (emission at 590 and 530 nm, respectively, with excitation at 490 nm) using a fluorescence microplate reader (Spectra Max i3x, Molecular Devices Inc. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. Data Analysis. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The chamber. Find regional support. Get Pricing. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. SeeSpectraMax MiniMaxImagingCytometeronpage85. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Explore a wealth of applications with the future-ready SpectraMax i3x. AquaMax Microplate Washer. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. 蛍光 発光 ELISA. each new assay. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. The assay can accurately test the toxicology of compounds as shown in Figure 1.